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The Effect of Thyme (Thymus vulgaris) Extract on the Expression of norA Efflux Pump Gene in Clinical Strains of Staphylococcus aureus | ||
| Journal of Genetic Resources | ||
| مقاله 4، دوره 4، شماره 1، خرداد 2018، صفحه 26-36 اصل مقاله (326.61 K) | ||
| نوع مقاله: Research Article | ||
| شناسه دیجیتال (DOI): 10.22080/jgr.2018.13900.1099 | ||
| نویسندگان | ||
| Ali Salehzadeh* 1؛ Mahsa Sadat Hashemi Doulabi1؛ Bita Sohrabnia1؛ Amir Jalali2 | ||
| 1Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran | ||
| 2Young Researchers and Elite Club, Rasht Branch, Islamic Azad University, Rasht, Iran | ||
| تاریخ دریافت: 04 شهریور 1396، تاریخ بازنگری: 16 مرداد 1397، تاریخ پذیرش: 18 مهر 1396 | ||
| چکیده | ||
| The NorA efflux pump considered as one of the contributors to antibiotic resistance in Staphylococcus aureus strains. One of the challenges of the researchers is finding natural plant compounds with the ability to inhibit the pumps. The aim of this study was to investigate the inhibitory effect of thyme (Thymus vulgaris) extract on NorA efflux pump in ciprofloxacin-resistant strains of S. aureus. Here, by using polymerase chain reaction (PCR), the presence of norA efflux pump was identified in 10 clinical and standard ciprofloxacin-resistant strains of S. aureus. The extract of thyme (T. vulgaris) was prepared using ethanol solvent and the effect of the extract against norA efflux pump was investigated by ethidium bromide method. In the following, after exposure to sub minimum inhibitory concentration of the extract, norA gene expression was evaluated using real-time polymerase chain reaction. Finally, we used the gas chromatography mass spectrometry (GC-mass) method to characterize the compounds with antibacterial properties. The results of PCR showed that all strains had a norA efflux pump, and ethidium bromide phenotypic method indicated that thyme extract had an inhibitory effect on all resistant strains with norA efflux pump. The norA gene expression in ciprofloxacin-resistant strains also decreased in sub minimum inhibitory concentration of the extract. By using gas chromatography- mass spectrometric, five chemical compounds of thymol, carvacrol, indole, 2-methoxy-4-methylphenol and quinic acid were determined as the dominant compounds of thyme (T. vulgaris) extract. Due to the antiefflux pump effect of thyme extract, it seems that this plant extract can be used as a good antibacterial component in the pharmaceutical industry of Iran. | ||
| کلیدواژه‌ها | ||
| Ciprofloxacin؛ Efflux pump؛ GC-mass؛ Staphylococcus aureus | ||
| مراجع | ||
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Chizzola R, Michitsch H, Franz C. 2008. Antioxidative properties of Thymus vulgaris leaves: comparison of different extracts and essential oil chemotypes. J Agric Food Chem 56: 6897-6904.
Chouhan S, Sharma K, Guleria S. 2017. Antimicrobial activity of some essential oils-present status and future perspectives. Medicines (Basel) 4(3): E58. doi: 10.3390/medicines4030058.
Cohen ML. 1992. Epidemiology of drug resistance: implications for a post-antimicrobial era. Science 257: 1050-1055.
Cosentino S, Tubeerso CIG, Pisano B, Satta M, Mascia V, Arzedi E, et al. 1999. In-vitro antimicrobial activity and chemical composition of sardinian Thymus essential oils. Lett Appl Microbiol 29: 130-135.
Costa SS, Falcão C, Viveiros M, Machado D, Martins M, Melo-Cristino J, et al. 2011. Exploring the contribution of efflux on the resistance to fluoroquinolones in clinical isolates of Staphylococcus aureus. BMC Microbiol 11: 241.
Costa SS, Junqueira E, Palma C, Viveiros M, Melo-Cristino J, Amaral L, et al. 2013. Resistance to antimicrobials mediated by efflux pumps in Staphylococcus aureus. Antibiotics (Basel) 2(1): 83-99.
Couto I, Costa SS, Viveiros M, Martins M, Amaral L. 2008. Efflux-mediated response of Staphylococcus aureus exposed to ethidium bromide. J Antimicrob Chemoth 62(3): 504-13.
Czaplewski L, Bax R, Clokie M, Dawson M, Fairhead H, Fischetti VA, et al. 2016. Alternatives to antibiotics: a pipeline portfolio review. Lancet Infect Dis. 16(2): 239-251.
Deans SG, Ritchie GA. 1987. Antimicrobial properties of plant essential oils. Int J Food Microbiol 5: 165-180.
De Kievit TR, Parkins MD, Gillis RJ, Srikumar R, Ceri H, Poole K, et al. 2001. Multidrug efflux pumps: expression patterns and contribution to antibiotic resistance in Pseudomonas aeruginosa biofilms. Antimicrob Agents Chemother 45(6): 1761-70.
Deryabin DG, Tolmacheva AA. 2015. Antibacterial and anti-quorum sensing molecular composition derived from Quercus cortex (Oak bark) extract. Molecules 20(9): 17093-17108.
Ding Y, Onodera Y, Lee JC, Hooper DC. 2008. NorB, an efflux pump in Staphylococcus aureus strain MW2, contributes to bacterial fitness in abscesses. J Bacteriol 190(21): 7123-7129.
Ghebleh N, Sadat Naghavi N. 2014. Antagonistic activity of bioactive compounds extracted from cyanobacterium Oscillatoria isolated from oil refinery waste. Int J Mol Clin Microbiol 4 (2): 457-462.
Gislene GF, Paulo C, Giuliana L. 2000. Antibacterial activity of plant extracts and phytochemicals on antibiotic resistant bacteria. Braz J Microbiol 31: 314–325.
Gohari AR, Saeidnia S, Mollazadeh K, Yassa N, Malmir M, Shahverdi AR. 2010. Isolation of a new quinic acid derivative and its antibacterial modulating activity. Daru 18(1): 69-73.
Grundmann H, Ires-de-Sousa M, Boyce J, Tiemersma E. 2006. Emer-gence and resurgence of meticillin-resistant Staphylococcus aureus as a public-health threat. Lancet 368: 874-885.
Heidari Z, Salehzadeh A, Sadat Shandiz SA, Tajdoost S. 2018. Anti‑cancer and anti‑oxidant properties of ethanolic leaf extract of Thymus vulgaris and its bio‑functionalized silver nanoparticles. 3 Biotech 8: 177. https://doi.org/10.1007/s13205-018-1199-x
Huet AA, Raygada JL, Mendiratta K, Seo SM, Kaatz GW. 2008. Multidrug efflux pump overexpression in Staphylococcus aureus after single and multiple in vitro exposures to biocides and dyes. Microbiology 154(10): 3144-3153.
Jansen AM, Cheffer JJC, Svendsen AB. 1987. Antimicrobial activity of essencial oils: a 1976-1986 literature review. Aspects of test methods. Planta Med 40: 395-398. Katz OT, Peled N, Yagupsky P. 2004. Evaluation of the current NCCLS guidelines for screening and confirming extended–spectrum beta-lactamase production in isolates of E. coli and Klebsiella species from bacteremic patients. Eur J Clin Microbiol Infect Dis 23: 813–817. Kosmidis C, Schindler BD, Jacinto PL, Patel D, Bains K, Seo SM, Kaatz GW. 2012. Expression of multidrug resistance efflux pump genes in clinical and environmental isolates of Staphylococcus aureus. Int J Antimicrob Agents 40: 204-209.
Kurlenda J, Grinholc M. 2012. Alternative therapies in Staphylococcus aureus diseases. Cta Biochim Pol 59(2): 171-184.
Le Loir Y, Baron F, Gautier M. 2003. Staphylococcus aureus and food poisoning. Genet Mol Res 2(1): 63-76.
Li XZ, Nikaido H. 2009. Efflux-mediated drug resistance in bacteria: an update. Drugs 69: 1555-1623.
Matsuda K, Toyoda H, Kakutani K, Hamada M, Ouchi S. 1990. Indole as an Antibacterial Substance against Pseudomonas solanacearum, Agric Biol Chem 54(11): 3039-3040.
Marchese A, Orhan IE, Daglia M, Barbieri R, Di Lorenzo A, Nabavi SF, et al. 2016. Antibacterial and antifungal activities of thymol: A brief review of the literature. Food Chem 210: 402-414
Mohadjerani M, Naqinezhad A, Aghasizadeh Sharbaf M. 2016. Platycladus orientalis Extracts with Antioxidant Activity from North of Iran. J Genet Resour 2 (2): 60-66.
Moustafa MFM, Alamri SA, Taha TH, Alrumman SA, 2013. In vitro antifungal activity of Argemoneochroleuca sweet latex against some pathogenic fungi. Afr J Biotechnol 12(10): 1132-1137.
Patil A, Jadhov V, 2014. GC-MS analysis of bioactive components from methanol leaf extract of Toddaliaasiatica (L.). Int J Pharm Sci Rev Res 29(1): 18-20.
Pourmand MR, Yousefi M, Salami SA, Amini M. 2014. Evaluation of expression of NorA efflux pump in ciprofloxacin-resistant Staphylococcus aureus against hexahydroquinoline derivative by real-time PCR. Acta Med Iran 52(6): 424-429.
Saiful AJ, Mastura M, Zarizal S, Mazurah MI, Shuhaimi M, Ali AM. 2008. Efflux genes and active efflux activity detection in Malaysian clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA). J Basic Microbiol 48(4): 245-251.
Savjani JK, Gajjar AK, Savjani KT. 2009. Mechanisms of resistance: useful tool to design antibacterial agents for drug-resistant bacteria. Mini-Rev Med Chem 9(2): 194-205.
Saxena G, McCutcheon AR, Farmer S, Towers GHN, Hancock REW. 1994. Antimicrobial constituents of Rhus glabra. J Ethnopharmacol 42: 95-99.
Sienkiewicz M, Lysakowska M, Denys P, Kowalczyk E. The antimicrobial activity of thyme essential oil against multidrug resistant clinical bacterial strains. Microb Drug Resist 18(2): 137-148.
Tintino SR, Oliveira-Tintino CD, Campina FF, Silva RL, Costa Mdo S, Menezes IR, et al. 2016. Evaluation of the tannic acid inhibitory effect against the NorA efflux pump of Staphylococcus aureus. Microb Pathog 97: 9-13.
Truong-Bolduc QC, Dunman PM, Strahilevitz J. Projan SJ, Hooper DC. 2005. MgrA is a multiple regulator of two new efflux pumps in Staphylococcus aureus. J Bacteriol 187: 2395-2405.
Tukmechi A, Ownagh A., Mohebbat A. 2010. In vitro antibacterial activities of ethanol extract of Iranian propolis (EEIP) against fish pathogenic bacteria (Aeromonas Hydroohila, Yersinia Ruckeri & Streptococcus Inae). Braz J Microbiol 41: 1086-1092.
Wayne PA. 2011. Clinical and laboratory standards Institute. In: Performance standards for antimicrobial susceptibility testing. 21th Inform Suppl 31(1): M100-S21.
Yoshida H, Bogaki M, Nakamura S, Ubukata K, Konno M. 1990. Nucleotide sequence and characterization of the Staphylococcus aureus norA gene, which confers resistance to quinolones. J Bacteriol 172: 6942-6949. | ||
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