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آثار شدت تمرینات تناوبی بر پروتئینهای مرتبط با دینامیک میتوکندری در قلب رتهای نر مبتلابه انفارکتوس میوکارد | ||
پژوهشنامه فیزیولوژی ورزشی کاربردی | ||
مقاله 12، دوره 14، شماره 28، بهمن 1397، صفحه 159-172 اصل مقاله (278.27 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22080/jaep.2019.13561.1718 | ||
نویسندگان | ||
ارسلان دمیرچی1؛ بابک عبادی* 2 | ||
1استاد فیزیولوژی ورزشی دانشکده علوم ورزشی دانشگاه گیلان، رشت، ایران | ||
2دانشجوی دکتری فیزیولوژی ورزش دانشکده تربیت بدنی و علوم ورزشی دانشگاه گیلان | ||
تاریخ دریافت: 10 بهمن 1396، تاریخ بازنگری: 05 اسفند 1396، تاریخ پذیرش: 23 اسفند 1396 | ||
چکیده | ||
مقدمه و هدف: انفارکتوس میوکارد (MI) شایعترین نوع بیماری قلبی می باشد. بر اساس مطالعات اخیر، اختلال در عملکرد میتوکندری در پیشرفت نارساییهای قلبی پذیرفتهشده است. فعالیت ورزشی نقش محافظتی در برابر بیماریهای قلبی عروقی دارد با این حال نقش پروتئینهای درگیر در داینامیک میتوکندری در پاسخ به فعالیت ورزشی بهخوبی مشخص نشده است. بنابراین، هدف پژوهش حاضر بررسی آثار شدت تمرینات تناوبی بر پروتئینهای مرتبط با داینامیک میتوکندری در قلب رتهای نر مبتلابه انفارکتوس میوکارد میباشد. موارد و روش ها: پژوهش حاضر، تجربی با طرح پسآزمون با گروه کنترل در رتهای ویستار 16 هفتهای بود. در این مطالعه، پس از ایجاد MI با بستن شریان کرونری(LAD) و اطمینان از ایجاد MI توسط اکوکاردیوگرافی، رتهای به گروههای تمرینی تناوبی باشدت بالا (HIIT)، متوسط (MIIT)، پایین (LIIT)، انفارکتوس میوکارد بیتمرین (MI-SED) و کنترل سالم (C) تقسیم شدند. 6 هفته پس از اجرای تمرینات ورزشی(5 جلسه در هفته، هرجلسه یک ساعت)، سطوح پروتئینهای MFN2، DRP1 و PGC-1α بافت قلب به روش وسترن بلات اندازهگیریوتحلیل دادهها با استفاده از آزمون ANOVA و تست تعقیبی بونفرونی در سطح معنی داری 05/0P≤ انجام شد. یافته ها: نتایج تحلیل داده ها نشان داد که سطوح پروتئین های MFN2 و PGC-1α در گروه MI-SED نسبت به کنترل سالم کاهش و سطوح پروتئنی DRP1 افزایش معنیداری داشت (05/0P≤). همچنین، سطوح پروتئینهای MFN2 و PGC-1α تنها در گروه MIIT نسبت به گروه MI-SED افزایش معنی داری داشت (05/0P≤) و بین سایر گروهها تفاوت معنی دار وجود نداشت (05/0P≤). با اینحال کاهش سطوح پروتئینی DRP1 در تمامی گروههای تمرینی نسبت به MI-SED معنیداری بود (05/0P≤) ولی تفاوتی بین سه شدت مختلف تمرین وجود نداشت (05/0P≤). نتیجه گیری: تمرین تناوبی با شدت متوسط (MIIT) منجر به بهبود پروتئینهای همجوشی و شکافت میتوکندری در رتهای مبتلا به انفارکتوس میوکارد میشود.در حالیکه تمرین تناوبی با شدت بالا و پایین (HIIT،LIIT) علی رغم کاهش معنی دار DRP-1، منجر به نغییرات قابل توجهی در محتوی پروتئینی MFN2 و PGC-1α نشد. | ||
کلیدواژهها | ||
انفارکتوس میوکارد؛ تمرین تناوبی؛ شدت فعالیت ورزشی؛ DRP1؛ MFN2T؛ PGC-1α؛ MFN2 | ||
عنوان مقاله [English] | ||
The effects of the intensity of interval training on mitochondrial dynamics-related proteins in the heart of male rats with myocardial infarction | ||
نویسندگان [English] | ||
Arsalan Damirchi1؛ Babak Ebadi2 | ||
1. | ||
2Cardiovascular Exercise Physiology Department, Faculty of Exercise Science, University of Guilan, Iran. | ||
چکیده [English] | ||
Introduction and Purpose: Myocardial infarction (MI) is the most common type of heart disease. According to recent studies, mitochondrial dysfunction has been accepted in the development of heart failure. Exercise training plays a protective role against cardiovascular disease. However, the role of proteins involved in mitochondrial dynamics is not understood in response to exercise training. Therefore, the aim of the present study was to investigate the effects of the intensity of interval training on mitochondrial dynamic proteins in the heart of male rats with myocardial infarction. Materials and Methods: The present study was performed with experimental posttest design method with control group in 16 week wistar rats. In this study, after MI with ligation of the left anterior descending coronary artery (LAD) and ensuring the creation of MI by echocardiography, rats were subjected to HIIT, MIIT and (LIIT training, Sham (MI-SED) and control (C). Six weeks after exercise, the levels of MFN2, DRP1 and PGC-1α proteins were measured by western blot . Results: data analysis showed that proteins level of MFN2 and PGC-1α decreased significantly in MI-SED group compared to healthy control and DRP1 protein levels increased significantly (P≤0.05). Also, MFN2 and PGC-1α proteins increased in MIIT compared with MI-SED and DRP1 protein levels decreased as well (P≤0.05). Discussion and conclusion: Moderate-intensity interval training (MIIT) leads to improved mitochondrial fusion and fusion proteins in rats with myocardial infarction. While high and low intensity interval training, despite increasing MFN2 and PGC-1α and reducing DRP1, failed to improve fusion and mitochondrial fission. | ||
کلیدواژهها [English] | ||
Myocardial Infarction, interval Training, Exercise Intensity, DRP1, MFN2T, PGC-1α, MFN2 | ||
مراجع | ||
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