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Evaluation of Genetic Diversity in Iranian Violet (Viola spp) Populations Using Morphological and RAPD Molecular Markers | ||
| Journal of Genetic Resources | ||
| دوره 6، شماره 2، 2020، صفحه 157-171 اصل مقاله (1.25 M) | ||
| نوع مقاله: Research Article | ||
| شناسه دیجیتال (DOI): 10.22080/jgr.2020.18739.1190 | ||
| نویسندگان | ||
| Samira Abolghasemi* ؛ Roohangiz Naderi؛ Mohammadreza Fattahi Moghadam | ||
| Department of Horticultural Sciences, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| تاریخ دریافت: 24 خرداد 1399، تاریخ بازنگری: 01 شهریور 1399، تاریخ پذیرش: 24 خرداد 1399 | ||
| چکیده | ||
| Recognition of genetic reserves and desirable genes is the basis of breeding programs. So far, in Iran, due to the lack of recognition of genetic resources, a considerable breeding program has not been done on native plants. The study of the genetic diversity of violets as a native plant with ornamental and medicinal uses is the great importance in advancing the breeding goals of this plant. So, in the present study, nine populations of Viola spp . from different regions of Iran were used for evaluation of inter and intra-population genetic diversity with RAPD marker, and eight populations of them were used to evaluate morphological, vegetative, and reproductive characteristics. From 11 used primers, 145 bands which showed high resolution and their length was between 250 to 3000 base pairs, were counted and used for RAPD analysis. According to the cluster analysis using the JACCARD similarity coefficient and UPGMA method, significant differences were found among populations. Molecular analysis of variance showed 77 and 23% inter and intra population genetic diversity, respectively. Principal component analysis classified effective characteristics in 6 groups which justified 89.62% of total changes and in the cluster analysis of morphological traits, populations were classified into three groups in distance 10. The results of our molecular and morphological analysis showed considerable diversity among violet populations in Iran, which can be used in future breeding programs. | ||
| کلیدواژه‌ها | ||
| DNA extraction؛ Classification؛ Cluster analysis؛ Molecular variance؛ UPGMA | ||
| مراجع | ||
|
Auge H, Neuffer B, Erlinghagen F, Grupe R, Brandl R. 2001. Demographic and random amplified polymorphic DNA analyses reveal high levels of genetic diversity in a clonal violet. Mol Ecol 10: 1811-1819.
Ballard HE, Sytsma KJ, Kowal RR. 1999. Shrinking the violets: phylogenetic relationships of infrageneric groups in Viola (Violaceae) based on internal transcribed spacer DNA sequences. Syst Bot 23: 439-458.
Becker W. 1910. Die Violen der Schweiz. Denkschr. Schweiz. Naturforsch Ges 45: 1-82.
Becker W. 1925. Viola L. Die natürlichen Pflanzenfamilien 21: 363-376.
Colagar AH, Yusefi M, Zarei M, Yousefzadeh H. 2013. Assessment of genetic diversity of Tilia rubra DC. by RAPD analysis in the Hyrcanian forests, north of Iran. Pol J Ecol 61: 341-348.
Culley TM, Sbita SJ, Wick A. 2007. Population genetic effects of urban habitat fragmentation in the Perennial Herb Viola pubescens (Violaceae) using ISSR Markers. Ann Bot 100: 91-100.
Doyle JJ, Doyle JL, Brown AH, Grace JP. 1990. Multiple origins of polyploids in the Glycine tabacina complex inferred from chloroplast DNA polymorphism. Proc Natl Acad Sci 87: 714-717.
Drozdova I, Bubenchikov R. 2004. Antioxidant activity of Viola odorata L. and Fragaria vesca L. polyphenolic complexes. Rastite Res 40: 92-96.
Eckestein R, Neill R, Danihelka J, Otte A, Hler W. 2006.Genetic structure among and within peripheral and central populations of three endangered floodplain violets. Mol Ecol 15: 2367-2379.
Farsi M, Zolali J. 2003. Principles of plant biotechnology. Translate Mashhad University Publication, p.495.
Gams H. 1926. Illustrierte Flora von Mitteleuropa. Band 5, Teil 1: Dicotyledones, Linaceae-Violaceae.
Guetrin WH, Bailey JP. 1970. Introduction to modern factor analysis. Ann Arbor, Mich, Edwards Brothers.
Hameric JL, Godt MJW. 1990. Allozyme diversity in plant species. The USA. Sinauer Associates Inc. 43-63.
Hodalova I, Mereda P, Martonfi P, Martonfiova L, Danihelka J. 2008. Morphological characters useful for the delimitation of taxa within Viola subsect. Viola (Violaceae): a morphometric study from the west Carpathians. Foli Geob 43: 83-117.
Jannatdoust M, Darvishzadeh R, Ebrahomi MA .2014. Studying genetic diversity in confectionery sunflower (Helianthus annuus l.) by using microsatellite markers. Plant Biotechnol. 6: 61-72.
Karimi HA. 2002. A dictionary of Iran’s vegetation plants. Tehran, Parcham Publisher. 8:3-6.
Khatamsaz M. 1991. 'Violaceae'. Ministry of Agriculture-Research Organization of Agriculture and Natural Resources-Research Institute of Forests and Rang.
Kimura M, Crow JF. 1963. The measurement of effective population number. Evolution 17: 279-288.
Koohgard M, Shiran B, Mirakhorli N. 2011. Study of genetic diversity between and within populations of Fritillaria imperialis in Zagrose regions using RAPD markers and implication for its conservation. Mod Gen 7: 353-362. (In Persian).
Lewontin RC. 1972. The apportionment of human diversity. Evolutionary Biology, New York, Springer, NY, 381-398.
Marcussen T. 2003. Evolution, phylogeography, and taxonomy within the Viola alba complex (Violaceae). Plant Syst Evol 237: 51-74.
Marcussen T. 2006. Allozymic variation in the widespread and cultivated Viola odorata (Violaceae) in western Eurasia. Bot J Linn Soc 151: 563-571.
Marcussen T, Borgen L, Nordal I. 2005. New distributional and molecular information call into question the systematic position of the West Asian Viola sintenisii (Violaceae). Bot J Linn Soc 147: 91-98.
Marcussen T, Borgen L. 2011. Species delimitation in the Ponto-Caucasian Viola sieheana complex, based on evidence from allozymes, morphology, ploidy levels, and crossing experiments. Plant Syst Evol 291: 183-196.
Marcussen T, Nordal I. 1997. Viola suavis, a new spices in the Nordic Flora, with analysis of the relation to other species in the subsection Viola (Vioalceae). Nordic J Bot 18: 221-237.
Mereda JrP, Hodalova I, Martonfi P, Kucera G, Lihova J. 2008. Intraspecific variationin Viola suavis in Europe: parallel evaluation of white-flowered morphotypes. Ann Bot 102: 443-462.
Mereda PJr, Hodalova I, Kucera J, Zozomova-Lihova JR, Letz D, Slovak M.2011. Genetic and morphological variation in Viola suavis s.l. (Violaceae) in the western Balkan Peninsula: twoendemic subspecies revealed. Syst Bio 9: 211-231.
Mohammadi SA, Prasanna BM. 2003. Analysis of genetic diversity in crop plants salient statistical tools and considerations. Cro Sci 43: 1235-1248.
Mozafarian V. 1996. A dictionary of Iranian plant names. Tehran: Farhang Moaser, 396
Nie M. 1973. Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci 70: 3321-3323.
Oh BJ, Ko MK, Lee CH. 1998. Identification of the series-specific random amplified polymorphic DNA markers of Viola species. Plant Bre 117: 295-296.
Shokrpour M, Mohammadi SA, Moghaddam M, Ziai SA, Javanshir A. 2008. Analysis of morphologic association, phytochemical and AFLP markers in milk thistle (Silybum marianum L.). J Appl Res Med Aromat Plants 24: 278-292.
Smith JSC, Smith OS. 1992. Fingerprinting crop varieties. Adv Agron 47: 140-149.
Tutin TG, Heywood VH, Burges NA, Valentine DH, Walters SM, Webb DA. 1964. Flora Europaea. Vol. 1. Lycopodiaceae to Platanaceae. Flora Europaea. Vol. 1. Lycopodiaceae to Platanaceae.
Vojdni P. 1993. Role of gene bank and plant genetic materials in increasing crop. In Proceedings of the first congress agronomy and plant breeding.
Weir BS. 1990. Genetic data analysis-methods for discrete population genetic data. Sinauer Associates.
Williams JGK, Kubelik AE, Livak KJ, Rafaiski JA, Tingey SC. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nuc Acid Res 18: 6531-6536.
Yavari AR, Nazeri V, Sefidkon F, Zamani Z, Hassani ME .2012. Evaluation of genetic diversity among and within some endemic populations of Thymus migricus Klokov & Desj-Shost using RAPD molecular markers. Iran J Med Aro Plants 28: 37-48.
Yockteng R, Ballard HE, Mansion G, Dajoz I, Nadot S. 2003. Relationships among pansies (Viola section Melanium) investigated using ITS and ISSR markers. Plant Sys Evo 241: 153-170. | ||
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