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Evaluation of Antifungal Activity of Defensin (Tfgd2) Using Its Heterologous Expression in E. coli | ||
| Journal of Genetic Resources | ||
| دوره 7، شماره 1، 2021، صفحه 116-124 اصل مقاله (451.33 K) | ||
| نوع مقاله: Research Article | ||
| شناسه دیجیتال (DOI): 10.22080/jgr.2021.20205.1227 | ||
| نویسندگان | ||
| Shiva Shiehbeiki؛ Mostafa Motallebi* ؛ Mohammad Reza Zamani؛ Esmat Jourabchi | ||
| Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran | ||
| تاریخ دریافت: 25 آبان 1399، تاریخ بازنگری: 17 دی 1399، تاریخ پذیرش: 12 آبان 1399 | ||
| چکیده | ||
| Defensins are a superfamily of antimicrobial peptides that can inhibit the growth of a broad spectrum of fungi. To evaluate the antifungal activity of Tfgd2 gene of Trigonella foenum graecum, the coding region of this gene in the cDNA form was subcloned in the expression vector pET26b (+) and the construct was designated as pETSH1. The obtained construct expressing the recombinant protein with a hexahistidine tag at the C-terminal end transformed into the E. coli BL21 (DE3). Taguchi test applied for optimizing the protein expression and the expressed protein TFGD2 confirmed by SDS-PAGE and western blotting. The recombinant TFGD2 protein was purified using affinity chromatography with a Ni-NTA column. In vitro assay indicated a broad spectrum of antifungal activity of purified expressed recombinant TFGD2 against different fungal phytopathogens, such as Fusarium oxysporum, Rhizoctonia solani, Sclerotinia sclerotiorum, Alternaria solani, and Verticillium dahliae. | ||
| کلیدواژه‌ها | ||
| Antifungal Activity؛ Defensin؛ SYTOX Green؛ TFGD2؛ Pathogenesis-related protein | ||
| مراجع | ||
|
Brook M, Tomlinson GH, Miles K, Smith RWP, Rossi AG, Gray M. 2016. Neutrophil-derived alpha defensins control inflammation by inhibiting macrophage mRNA translation. Proc Natl Acad Sci 113: 4350-4355.
Campos MA, Silva MS, Magalhães CP, Ribeiro SG, Sarto RP, de Sá MF. 2008. Expression in Escherichia coli, purification, refolding and antifungal activity of an osmotin from Solanum nigrum. Microb Cell Fact 7: 7-10.
Carvalho Ade O, Gomes VM. 2009. Plant defensins--prospects for the biological functions and biotechnological properties. Peptides 30: 1007-1020.
Carvalho Ade O, Gomes VM. 2011. Plant defensins and defensin-like peptides- biological activities and biotechnological applications. Curr Pharm Des 17: 4270-4293.
Chen H, Xu Z, Xu N, Cen P. 2005. Efficient production of a soluble fusion protein containing human beta-defensin-2 in E. coli cell-free system. J Biotechnol 115: 307-315.
Chowdhury S, Basu A, Kundu, S. 2015. Cloning, characterization, and bacterial over-expression of an osmotin-like protein gene from Solanum nigrum L. with antifungal activity against three necrotrophic fungi. Mol Biotechnol 57(4): 371-381.
De Beer A, Vivier MA. 2011. Four plant defensins from an indigenous South African Brassicaceae species display divergent activities against two test pathogens despite high sequence similarity in the encoding genes. BMC Res Notes 4: 1-19
De Coninck B, Cammue BPA, Thevissen K. 2013. Modes of antifungal action and in planta functions of plant defensins and defensin-like peptides. Fungal Biol Rev 26: 109-120.
Etebari M, Motallebi M, Zamani M, Moghaddassi Jahromi Z. 2016. Structural Analysis of Def2 Gene from Trigonella foenum-graecum by Characterization of Its Genomic and cDNA. Agric Biotechnol J 7: 79-86.
Hu X, Reddy ASN. 1997. Cloning and expression of a PR5-like protein from Arabidopsis: inhibition of fungal growth by bacterially expressed protein. Plant Mol 34: 949-959.
Karri V, Bharadwaja KP. 2013. Tandem combination of Trigonella foenum-graecum defensin (Tfgd2) and Raphanus sativus antifungal protein (RsAFP2) generates a more potent antifungal protein. Funct Integr Genomics 13: 435-443.
Kirubakaran SI, Begum SM, Ulaganathan K, Sakthivel N. 2008. Characterization of a new antifungal lipid transfer protein from wheat. Plant Physiol Bioch 46: 918-927.
Kushmerick C, de Souza Castro M, Santos Cruz J, Bloch C, Beirão PSL. 1998. Functional and structural features of γ-zeathionins, a new class of sodium channel blockers. FEBS Lett 440: 302-306.
Larentis AL, Nicolau JF, dos Santos Esteves G, Vareschini DT, de Almeida FV, Medeiros MA. 2014. Evaluation of pre-induction temperature, cell growth at induction and IPTG concentration on the expression of a leptospiral protein in E. coli using shaking flasks and microbioreactor. BMC Res Notes 7:1-13.
Lay FT, Anderson MA. 2005. Defensins--components of the innate immune system in plants. Curr Protein Pept Sci 6: 85-101.
Li R, Wang M, Wang Y, Schuman MC, Weinhold A, Baldwin IT. 2017. Flower-specific jasmonate signaling regulates constitutive floral defenses in wild tobacco. Proc Natl Acad Sci 114: E7205-E7214.
Low KO, Jonet MA, Ismail NF, Illias RM. 2012. Optimization of a Bacillus sp signal peptide for improved recombinant protein secretion and cell viability in Escherichia coli: Is there an optimal signal peptide design? Bioengineered 3:334-338.
Makrides SC. 1996. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol Rev 60:512-538.
Mirouze M, Sels J, Richard O, Czernic P, Loubet S, Marquès L. 2006. A putative novel role for plant defensins: a defensin from the zinc hyper-accumulating plant, Arabidopsis halleri, confers zinc tolerance. Plant J 47:329-342.
Mith O, Benhamdi A, Castillo T, Berge M, MacDiarmid CW, Berthomieu P. 2015. The antifungal plant defensin Ah PDF 1.1 b is a beneficial factor involved in adaptive response to zinc overload when it is expressed in yeast cells. Microbiologyopen 4:409-422.
Olli S, Kirti PB. 2006. Cloning, characterization and antifungal activity of defensin Tfgd1 from Trigonella foenum-graecum L. J Biochem Mol Biol 39: 278-283.
Papaneophytou CP, Kontopidis G. 2014. Statistical approaches to maximize recombinant protein expression in Escherichia coli: a general review. Protein Expr Purif 94:22-32.
Pelegrini PB, Lay FT, Murad AM, Anderson MA, Franco OL. 2008. Novel insights on the mechanism of action of alpha-amylase inhibitors from the plant defensin family. Protein 73: 719-729.
Pellegrini A. 2003. Antimicrobial peptides from food proteins. Curr Pharm Des 9: 1225-1238.
Picart P, Pirttilä AM, Raventos D, Kristensen HH, Sahl HG. 2012. Identification of defensin-encoding genes of Picea glauca: characterization of PgD5, a conserved spruce defensin with strong antifungal activity. BMC plant Biology 12(1): 1-14.
Silaban S, Gaffar S, Simorangkir M, Maksum IP, Subroto T. 2018. Effect of IPTG concentration on recombinant human prethrombin-2 expression in Escherichia coli BL21 (DE3) ArcticExpress. InIOP Conference Series: Earth Environ Sci 217: p 012039.
Singh P, Sharma L, Kulothungan SR, Adkar BV, Prajapati RS, Varadarajan R. 2013. Effect of signal peptide on stability and folding of Escherichia coli thioredoxin. PLoS One 8: e63442.
Spelbrink RG, Dilmac N, Allen A, Smith TJ, Shah DM, Hockerman GH. 2004. Differential Antifungal and Calcium Channel-Blocking Activity among Structurally Related Plant Defensins. Plant Physiol 135: 2055- 2067.
Stotz HU, Thomson J, Wang Y. 2009. Plant defensins: defense, development and application. Plant Signal Behav 4: 1010-1012.
Thevissen K, Ghazi A, De Samblanx GW, Brownlee C, Osborn RW, Broekaert WF. 1996. Fungal Membrane Responses Induced by Plant Defensins and Thionins. J Biol Chem 271: 15018-15025.
Vi TX, Le HD, Nguyen VT, Van Son LE, Chu HM. 2017. Expression of the ZmDEF1 gene and α-amylase inhibitory activity of recombinant defensin against maize weevils. Turk J Biol 41:98-104.
Volontè F, Marinelli F, Gastaldo L, Sacchi S, Pilone MS, Molla G. 2008. Optimization of glutaryl-7-aminocephalosporanic acid acylase expression in E. coli. Protein Expr Purif 61: 131-137.
Vriens K, Cammue BP, Thevissen K. 2014. Antifungal plant defensins: mechanisms of action and production. Molecules 19: 12280-12303.
Wilmes M, Cammue BP, Sahl HG, Thevissen K. 2011. Antibiotic activities of host defense peptides: more to it than lipid bilayer perturbation. Nat Prod Rep 28: 1350-1358.
Xiang F, Xie Z, Feng J, Yang W, Cao Z, Wu Y. 2015. Plectasin, first animal toxin-like fungal defensin blocking potassium channels through recognizing channel pore region. Toxins 7: 34-42. | ||
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