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Gene Expression Analysis of Inflammation, Proliferation, and Tissue Repair in Fibroblasts Treated with Ishtx-k Toxin and Mucilage | ||
| Journal of Genetic Resources | ||
| دوره 12، شماره 1، 2026، صفحه 74-83 اصل مقاله (715.17 K) | ||
| نوع مقاله: Research Article | ||
| شناسه دیجیتال (DOI): 10.22080/jgr.2026.31890.1464 | ||
| نویسندگان | ||
| Atefeh Khakdan؛ Hoda Ayat؛ Ali Mohammad Ahadi* | ||
| Department of Genetics, Faculty of Science, Shahrekord University, Shahrekord, Iran | ||
| تاریخ دریافت: 06 آذر 1404، تاریخ بازنگری: 27 خرداد 1405، تاریخ پذیرش: 08 دی 1404 | ||
| چکیده | ||
| The present study was conducted to investigate the effects of a combination derived from okra extract and the sea anemone toxin Ishtx-K under cell culture conditions. Fibroblasts are one of the most important cells involved in tissue repair. Modulation of fibroblast growth rate is useful for wound-healing management. In this study, in order to more precisely evaluate alterations in gene expression under in vitro conditions, cell cultures treated with mucilage and the Ishtx-K peptide were employed. Real-time RT-PCR assays were performed to analyze the expression changes of key genes associated with inflammation and tissue repair, including FGF1, TAC3, NF-κB, and the two long non-coding transcripts H19 and NEAT1. Statistical analysis was performed in the P≤ 0.05 significant range. The results demonstrated that treatment with okra mucilage and the Ishtx-K peptide reduced the expression of NF-Κb (fold change/FC= 0.47, P= 0.000), one of the most important regulators of inflammation. Significant alterations were also observed in the expression of FGF1 (overexpression to 4.2-fold, P= 0.000) and a decrease of about 50% in TAC3 in the group treated with mucilage, indicating stimulation of regenerative processes and modulation of pathways associated with tissue repair. In addition, diminished expression changes of the non-coding transcripts H19 and NEAT1, confirmed their potential roles in the molecular mechanisms underlying cell signaling related to cell growth and cell physiology, were FC= 0.2-0.25 in the mucilage-treated group for two molecules, P= 0.000 and about 0.39 for LncRNA NEATA1 in the mucilage-toxin combined treated group, P= 0.000. The combined application of the plant-derived and peptide-based compounds exhibited greater efficacy in reducing inflammation and improving the regenerative process compared with individual treatments. These findings suggest that the combined therapy presented in this study, through modulation of inflammatory and molecular pathways, may represent a novel and effective therapeutic approach for some important process in tissue repair. | ||
| کلیدواژهها | ||
| Recombinant Ishtx-K peptide؛ Okra mucilage؛ Gene expression؛ Cell proliferation | ||
| مراجع | ||
|
Afsharian, Y. P., & Rahimnejad, M. (2021). Bioactive electrospun scaffolds for wound healing applications: A comprehensive review. Polymer Testing, 93, 106952. https://doi.org/10.1016/j.polymertesting.2020.106952
Berry, C. E., Brenac, C., Gonzalez, C. E., Kendig, C. B., Le, T., An, N., & Griffin, M. F. (2024). Natural compounds and biomimetic engineering to influence fibroblast behavior in wound healing. International Journal of Molecular Sciences, 25(6), 3274. https://doi.org/10.3390/ijms25063274
Chang, S. C., Bajaj, S., & Chandy, K. (2018). ShK toxin: history, structure and therapeutic applications for autoimmune diseases. Wiki Journal of Science, 1(1), 1-13. https://doi.org/10.15347/wjs/2018.003
Chang, Y., Lee, J. W. N., & Holle, A. W. (2025). The mechanobiology of fibroblast activation in disease. Applied Physics Letters Bioengineering, 9(2), 021505. https://doi.org/10.1063/5.0272393
Correa‐Gallegos, D., Jiang, D., & Rinkevich, Y. (2021). Fibroblasts as confederates of the immune system. Immunological Reviews, 302(1), 147-162. https://doi.org/10.1111/imr.12972
Coughlin, M. L., Liberman, L., Ertem, S. P., Edmund, J., Bates, F. S., & Lodge, T. P. (2021). Methyl cellulose solutions and gels: fibril formation and gelation properties. Progress in Polymer Science, 112, 101324. https://doi.org/10.1016/j.progpolymsci.2020.101324
De Domenico, S., La Banca, V., D’Amico, S., Nicolai, S., & Peschiaroli, A. (2025). Defining the transcriptional routes controlling lncRNA NEAT1 expression: implications in cellular stress response, inflammation, and differentiation. Discover Oncology, 16(1), 768. https://doi.org/ 10.1007/s12672-025-02510-6
De Oliveira Filho, J. G., Lira, M. M., de Sousa, T. L., Campos, S. B., Lemes, A. C., & Egea, M. B. (2021). Plant based mucilage with healing and anti-inflammatory actions for topical application: A review. Food Hydrocolloids for Health, 1, 100012. https://doi.org/10.1016/j.fhfh.2021.100012
Elkhalifa, A. E. O., Alshammari, E., Adnan, M., Alcantara, J. C., Awadelkareem, A. M., Eltoum, N. E., Ashraf, S. A. (2021). Okra as a potential dietary medicine with nutraceutical importance for sustainable health applications. Molecules, 26(3), 696. https://doi.org/10.3390/molecules26030696
Farooq, M., Khan, A. W., Kim, M. S., & Choi, S. (2021). The role of fibroblast growth factor (FGF) signaling in tissue repair and regeneration. Cells, 10(11), 3242. https://doi.org/10.3390/cells10113242
Frazao, B., Vasconcelos, V., & Antunes, A. (2012). Sea anemone (cnidaria, anthozoa, actiniaria) toxins: An overview. Marine Drugs, 10(8), 1812-1851. https://doi.org/10.3390/md10081812
Guo, S. A., & DiPietro, L. A. (2010). Factors affecting wound healing. Journal of Dental Research, 89(3), 219-229. https://doi.org/10.1177/0022034509359125
Guo, Y., Gao, F., Rafiq, M., Yu, B., Cong, H., & Shen, Y. (2024). Preparation of antimicrobial peptides and their combination with hydrogels for wound healing applications. International Journal of Biological Macromolecules, 274, 133494. https://doi.org/10.1016/j.ijbiomac.2024.133494.
Ibrahim, N. I., Wong, S. K., Mohamed, I. N., Mohamed, N., Chin, K. Y., Ima-Nirwana, S., & Shuid, A. N. (2018). Wound healing properties of selected natural products. International Journal of Environmental Research and Public Health, 15(11), 2360. https://doi.org/10.3390/ijerph15112360.
Khorasani, S., Boroumand, N., Lavi Arab, F., & Hashemy, S. I. (2020). The immunomodulatory effects of tachykinins and their receptors. Journal of Cellular Biochemistry, 121(5-6), 3031-3041. https://doi.org/10.1002/jcb.29668
Kruger, N. J. (2009). The Bradford method for protein quantitation. The Protein Protocols Handbook, 17-24. https://doi.org/10.1385/0-89603-268-X:9
Li, B., Qian, L., Pi, L., & Meng, X. (2023). A therapeutic role of exosomal lncRNA H19 from adipose mesenchymal stem cells in cutaneous wound healing by triggering macrophage M2 polarization. Cytokine, 165, 156175. https://doi.org/10.1016/j.cyto.2023.156175.
Liu, D., Zhong, Z., & Karin, M. (2022). NF-κB: A double-edged sword controlling inflammation. Biomedicines, 10(6), 1250. https://doi.org/10.3390/biomedicines10061250
Moretti, L., Stalfort, J., Barker, T. H., & Abebayehu, D. (2022). The interplay of fibroblasts, the extracellular matrix, and inflammation in scar formation. Journal of Biological Chemistry, 298(2), 101530. https://doi.org/10.1016/j.jbc.2021.101530
Mu, X., Wang, H., & Li, H. (2021). Silencing of long noncoding RNA H19 alleviates pulmonary injury, inflammation, and fibrosis of acute respiratory distress syndrome through regulating the microRNA-423-5p/FOXA1 axis. Experimental Lung Research, 47(4), 183-197. https://doi.org/10.1080/01902148.2021.1887967
Shedoeva, A., Leavesley, D., Upton, Z., & Fan, C. (2019). Wound healing and the use of medicinal plants. Evidence Based Complementary and Alternative Medicine, 2019(1), 2684108. https://doi.org/10.1155/2019/2684108
Sipahi, H., Orak, D., Reis, R., Yalman, K., Senol, O., Palabiyik-Yucelik, S. S., ... & Yesilada, E. (2022). A comprehensive study to evaluate the wound healing potential of okra (Abelmoschus esculentus) fruit. Journal of Ethnopharmacology, 287, 114843. https://doi.org/10.1016/j.jep.2021.114843
Song, J., Li, X., & Li, J. (2020). Emerging evidence for the roles of peptide in hypertrophic scar. Life Sciences, 241, 117174. https://doi.org/ 10.1016/j.lfs.2019.117174
Utkin, Y. N. (2015). Animal venom studies: Current benefits and future developments. World Journal of Biological Chemistry, 6(2), 28. https://doi.org/ 10.4331/wjbc.v6.i2.28
Wang, F., Jiang, X., Zhao, Z., Wang, Y., Jiang, H., Gao, Y., ... & Li, Z. (2025). Neurokinin B is a potential target for treating disruption of intestinal mucosal barrier in acute mechanical intestinal obstruction. Peptides, 191, 171419. https://doi.org/10.1016/j.peptides.2025.171419
Yilmaz, H. D., Cengiz, U., Derkus, B., & Arslan, Y. E. (2023). Development of plant-based biopolymer coatings for 3D cell culture: boron-silica-enriched quince seed mucilage nanocomposites. Biomaterials Science, 11(15), 5320-5336. https://doi.org/10.1039/D3BM00170A
Zhang, X., Duan, X. J., Li, L. R., & Chen, Y. P. (2022). LncRNA NEAT1 promotes hypoxia‐induced inflammation and fibrosis of alveolar epithelial cells via targeting miR‐29a/NFATc3 axis. The Kaohsiung Journal of Medical Sciences, 38(8), 739-748. https://doi.org/10.1002/kjm2.12535 | ||
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