دانشگاه مازندران

 آمار

تعداد نشریات32
تعداد شماره‌ها581
تعداد مقالات5,665
تعداد مشاهده مقاله8,610,298
تعداد دریافت فایل اصل مقاله6,280,627
Boudaghpour, Siamak, Bahadori, Amirali, Qamari, Ehsan. (1405). Hydrolytic Enzyme Effects on Total Coliforms in Surfactant-Rich Industrial Wastewater and Soil Extracts: A Control-Adjusted Assessment. پژوهشنامه مدیریت اجرایی, (), 70-82. doi: 10.22080/ceas.2026.31906.1102
Siamak Boudaghpour; Amirali Bahadori; Ehsan Qamari. "Hydrolytic Enzyme Effects on Total Coliforms in Surfactant-Rich Industrial Wastewater and Soil Extracts: A Control-Adjusted Assessment". پژوهشنامه مدیریت اجرایی, , , 1405, 70-82. doi: 10.22080/ceas.2026.31906.1102
Boudaghpour, Siamak, Bahadori, Amirali, Qamari, Ehsan. (1405). 'Hydrolytic Enzyme Effects on Total Coliforms in Surfactant-Rich Industrial Wastewater and Soil Extracts: A Control-Adjusted Assessment', پژوهشنامه مدیریت اجرایی, (), pp. 70-82. doi: 10.22080/ceas.2026.31906.1102
Boudaghpour, Siamak, Bahadori, Amirali, Qamari, Ehsan. Hydrolytic Enzyme Effects on Total Coliforms in Surfactant-Rich Industrial Wastewater and Soil Extracts: A Control-Adjusted Assessment. پژوهشنامه مدیریت اجرایی, 1405; (): 70-82. doi: 10.22080/ceas.2026.31906.1102

Hydrolytic Enzyme Effects on Total Coliforms in Surfactant-Rich Industrial Wastewater and Soil Extracts: A Control-Adjusted Assessment

Civil Engineering and Applied Solutions
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 10 مرداد 1405
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22080/ceas.2026.31906.1102
نویسندگان
Siamak Boudaghpour* 1؛ Amirali Bahadori2؛ Ehsan Qamari1
1Department of Environmental Engineering, Faculty of Civil Engineering, K. N. Toosi University of Technology, Tehran, Iran
2Department of Water Resources Management, Faculty of Civil Engineering, K. N. Toosi University of Technology, Tehran, Iran
تاریخ دریافت: 04 خرداد 1405،  تاریخ بازنگری: 26 خرداد 1405،  تاریخ پذیرش: 10 مرداد 1405 
چکیده
Detergent manufacturing wastewater contains surfactants, organic contaminants, and microbial populations that may challenge conventional treatment processes. This study evaluated the response of total coliforms to hydrolytic enzyme treatments in detergent-impacted industrial wastewater and aqueous soil extracts under controlled laboratory conditions. Cellulase, amylase, protease, an enzyme-supplemented detergent formulation, an enzyme-free detergent formulation, and untreated controls were assessed during a 7-day incubation period. Total coliform abundance was determined using the five-tube Most Probable Number (MPN) method, and treatment performance was evaluated using endpoint abundance and residual coliform counts. A substantial decline occurred in the untreated wastewater control, which exhibited an 88.5% reduction in coliform abundance, indicating that natural attenuation represented a major component of the overall response. Among wastewater treatments, cellulase (WW-CEL) and the enzyme-supplemented detergent formulation (WW-D+E) exhibited the lowest endpoint coliform abundances, with endpoint values at or near the analytical detection limit. Relative to the untreated control, WW-CEL was associated with an additional reduction of approximately 11.4% beyond the baseline decline observed under the experimental conditions. In contrast, the enzyme-free detergent treatment produced an endpoint response comparable to the untreated control. Most soil extract treatments exhibited baseline coliform abundances at or below the analytical detection limit, limiting interpretation of treatment-specific responses. These findings suggest that hydrolytic enzymes may contribute to incremental reductions in coliform abundance in surfactant-rich industrial wastewater. However, the overall response was strongly dominated by natural attenuation, and observed treatment effects were highly dependent on matrix characteristics and baseline microbial load.
کلیدواژه‌ها
Enzymatic bioaugmentation؛ Industrial wastewater treatment؛ Coliform suppression؛ Cell envelope disruption؛ Surfactant synergism؛ Matrix-dependent attenuation
مراجع
  1. Mourão, J. M. M., da Costa, A. A. A., de Carvalho, I. E. B., dos Santos, A. B., Firmino, P. I. M. Impact of linear alkylbenzene sulfonate (LAS) on the performance of an aerobic granular sludge (AGS) reactor. Process Safety and Environmental Protection, 2026; 208: 108465. doi:10.1016/j.psep.2026.108465.
  2. Pan, H., Shi, J., Xu, D., Wang, J., Ma, Q. Environmental occurrence, ecological risks, and microbial interactions of p-chloro-m-xylenol: An emerging ubiquitous antimicrobial agent. International Biodeterioration & Biodegradation, 2026; 207: 106229. doi:10.1016/j.ibiod.2025.106229.
  3. Min, X.-Z., Zhang, Z.-F., Lu, X.-M., Chen, J.-C., Ma, W.-L., Liu, L.-Y., Li, W.-L., Li, Y.-F., Kallenborn, R. Occurrence and fate of pharmaceuticals and personal care products in a wastewater treatment plant with Bacillus bio-reactor treatment. Science of The Total Environment, 2024; 924: 171589. doi:10.1016/j.scitotenv.2024.171589.
  4. Wang, J., Jiao, D., Yuan, S., Chen, H., Dai, J., Wang, X., Guo, Y., Qiu, D. Comparative analysis of microbial community under acclimation of linear alkylbenzene sulfonate (LAS) surfactants and degradation mechanisms of functional strains. Journal of Hazardous Materials, 2024; 477: 135370. doi:10.1016/j.jhazmat.2024.135370.
  5. Lou, H., Cao, X. Quantifying the rate-limiting step and microbial stratification in linear alkylbenzene sulfonate biodegradation within a pilot-scale anaerobic-aerobic biofilm system. Biochemical Engineering Journal, 2026; 229: 110115. doi:10.1016/j.bej.2026.110115.
  6. Zhao, W., Chen, P. Resilient biological nitrogen removal from surfactant-rich wastewater: construction of an indigenous community, quantitative tolerance assessment, and multi-level fault tolerance mechanisms. Bioresource Technology, 2026; 447: 134251. doi:10.1016/j.biortech.2026.134251.
  7. Kong, W., Jalalah, M., Alsareii, S. A., Harraz, F. A., Almadiy, A. A., Zheng, Y., Thakur, N., Salama, E.-S. Microplastics (MPs) in wastewater treatment plants sludges: Substrates, digestive properties, microbial communities, mechanisms, and treatments. Journal of Environmental Chemical Engineering, 2023; 11: 111408. doi:10.1016/j.jece.2023.111408.
  8. Liu, S., Su, C., Lu, Y., Xian, Y., Chen, Z., Wang, Y., Deng, X., Li, X. Effects of microplastics on the properties of different types of sewage sludge and strategies to overcome the inhibition: A review. Science of The Total Environment, 2023; 902: 166033. doi:10.1016/j.scitotenv.2023.166033.
  9. Alvarez, L., Hernandez, S. B., Torrens, G., Weaver, A. I., Dörr, T., Cava, F. Control of bacterial cell wall autolysins by peptidoglycan crosslinking mode. Nature Communications, 2024; 15: 7937. doi:10.1038/s41467-024-52325-2.
  10. Schönbichler, A., Díaz-Moreno, S. M., Srivastava, V., McKee, L. S. Exploring the Potential for Fungal Antagonism and Cell Wall Attack by Bacillus subtilis natto. Frontiers in Microbiology, 2020; Volume 11: 521. doi:10.3389/fmicb.2020.00521.
  11. Ma, W., Lei, Y., Wang, L., Chen, Y., Ma, M., Chen, X. Bacteriophage Lysins as Programmable Antimicrobials: Mechanisms, Engineering, and Translational Advances. Probiotics and Antimicrobial Proteins, 2026; doi:10.1007/s12602-026-10966-3.
  12. Gharechahi, J., Vahidi, M. F., Sharifi, G., Ariaeenejad, S., Ding, X.-Z., Han, J.-L., Salekdeh, G. H. Lignocellulose degradation by rumen bacterial communities: New insights from metagenome analyses. Environmental Research, 2023; 229: 115925. doi:10.1016/j.envres.2023.115925.
  13. Zhu, G.-S., Yang, X.-X., Zhang, Y., Zhang, X.-Y., Lin, L.-B., Wang, F. Bacteriophage lysin P26ly combats multidrug-resistant Escherichia coli O157 and methicillin-resistant Staphylococcus aureus via cell wall degradation and membrane disruption. International Journal of Biological Macromolecules, 2026; 336: 149288. doi:10.1016/j.ijbiomac.2025.149288.
  14. Wu, S., Snajdrova, R., Moore, J. C., Baldenius, K., Bornscheuer, U. T. Biocatalysis: Enzymatic Synthesis for Industrial Applications. Angewandte Chemie International Edition, 2021; 60: 88–119. doi:10.1002/anie.202006648.
  15. Al-Madboly, L. A., Aboulmagd, A., El-Salam, M. A., Kushkevych, I., El-Morsi, R. M. Microbial enzymes as powerful natural anti-biofilm candidates. Microbial Cell Factories, 2024; 23: 343. doi:10.1186/s12934-024-02610-y.
  16. Thallinger, B., Argirova, M., Lesseva, M., Ludwig, R., Sygmund, C., Schlick, A., Nyanhongo, G. S., Guebitz, G. M. Preventing microbial colonisation of catheters: Antimicrobial and antibiofilm activities of cellobiose dehydrogenase. International Journal of Antimicrobial Agents, 2014; 44: 402–408. doi:10.1016/j.ijantimicag.2014.06.016.
  17. Dias-Souza, M. V., Alves, A. L., Pagnin, S., Veiga, A. A., Haq, I. U., Alonazi, W. B., dos Santos, V. L. The activity of hydrolytic enzymes and antibiotics against biofilms of bacteria isolated from industrial-scale cooling towers. Microbial Cell Factories, 2024; 23: 282. doi:10.1186/s12934-024-02502-1.
  18. Ramakrishnan, R., Singh, A. K., Singh, S., Chakravortty, D., Das, D. Enzymatic dispersion of biofilms: An emerging biocatalytic avenue to combat biofilm-mediated microbial infections. Journal of Biological Chemistry, 2022; 298: 102352. doi:10.1016/j.jbc.2022.102352.
  19. Wang, S., Zhao, Y., Breslawec, A. P., Liang, T., Deng, Z., Kuperman, L. L., Yu, Q. Strategy to combat biofilms: a focus on biofilm dispersal enzymes. npj Biofilms and Microbiomes, 2023; 9: 63. doi:10.1038/s41522-023-00427-y.
  20. Lara-Moreno, A., Vargas-Ordóñez, A., Villaverde, J., Madrid, F., Carlier, J. D., Santos, J. L., Alonso, E., Morillo, E. Bacterial bioaugmentation for paracetamol removal from water and sewage sludge. Genomic approaches to elucidate biodegradation pathway. Journal of Hazardous Materials, 2024; 480: 136128. doi:10.1016/j.jhazmat.2024.136128.
  21. Xian, Y., Cao, L., Lu, Y., Li, Q., Su, C., He, Y., Zhou, G., Chen, S., Gao, S. Metagenomics and metaproteomics reveal the effects of sludge types and inoculation modes on N, N-dimethylformamide degradation pathways and the microbial community involved. Journal of Hazardous Materials, 2025; 481: 136548. doi:10.1016/j.jhazmat.2024.136548.
  22. Qin, D., Chen, T., Adyari, B., Kiki, C., Sun, Q., Yu, C.-P. Responses of microbial community to the selection pressures of low-concentration contaminants of emerging concern in activated sludge. Journal of Hazardous Materials, 2025; 490: 137880. doi:10.1016/j.jhazmat.2025.137880.
  23. Baird, R., Eaton, A. D., Rice, E. W., Bridgewater, L., Association, A. P. H., Association, A. W. W., Federation, W. E. Standard Methods for the Examination of Water and Wastewater. 23th ed. Washington, D.C. (US): American Public Health Association; 2017.
آمار
تعداد مشاهده مقاله: 56


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب