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Temporal Regulation of Autophagy- and Mitophagy-related Genes in Saccharomyces cerevisiae under Gradual Glucose Restriction | ||
| Journal of Genetic Resources | ||
| دوره 12، شماره 2، 2026، صفحه 206-219 اصل مقاله (953.55 K) | ||
| نوع مقاله: Research Article | ||
| شناسه دیجیتال (DOI): 10.22080/jgr.2026.32509.1481 | ||
| نویسندگان | ||
| Hengameh Hoshyar-Chamanarae1؛ Madjid Momeni-Moghaddam* 1؛ Shatha Saadi Jumaah2 | ||
| 1Department of Biology, Hakim Sabzevari University, Sabzevar, Iran | ||
| 2University of Al- Hamdaniya, Education Faculty, Biology Department, Iraq | ||
| تاریخ دریافت: 13 خرداد 1405، تاریخ بازنگری: 16 شهریور 1405، تاریخ پذیرش: 12 تیر 1405 | ||
| چکیده | ||
| Nutrient availability is widely recognized as a critical regulator of cellular homeostasis and metabolic adaptation. In this study, we investigated the transcriptional dynamics of autophagy- and mitophagy-related genes in Saccharomyces cerevisiae under graded glucose restriction (1%, 0.5%, and 0.2%), using a 2% glucose environment as the control. Growth kinetics, metabolic vitality, and qualitative survival profiles were systematically evaluated on days 5 and 7, corresponding to critical chronological transition phases. Additionally, relative mRNA expression levels of ATG8, ATG11, and ATG32 were quantified via qRT-PCR. On day 5, a pronounced, time-dependent metabolic surge was observed through MTT assays across all restricted environments, correlating with enhanced colony-forming efficiency in spotting assays. Concurrently, transcriptional analyses revealed that severe glucose restriction (0.2%) induced significant up-regulation of the mitophagy receptor ATG32 and the scaffold protein ATG11. Furthermore, a substantial down-regulation of the core autophagy marker ATG8 was simultaneously detected across all restricted groups, suggesting that decreased ATG8 expression may reflect an overall reduction in general autophagic activity, while the concurrent induction of ATG32 and ATG11 indicates a localized retention or activation of selective mitochondrial quality control under extreme restriction. This selective transcriptional response suggests that S. cerevisiae possesses an acute, temporary adaptive capacity. By day 7, however, metabolic activity and transcriptional profiles had returned toward a baseline maintenance levels, with expression differences becoming statistically comparable to the control. Notably, overall cellular viability was successfully preserved throughout these nutritional adjustments. Consequently, this study demonstrates that S. cerevisiae has an acute, temporary adaptive capacity, whereby its autophagic machinery can be dynamically recalibrated to optimize energy expenditure and selectively manage organelle quality under prolonged nutritional stress. | ||
| کلیدواژه‌ها | ||
| ATG32 / ATG11 axis؛ Chronological lifespan؛ Glucose restriction؛ Metabolic flexibility؛ Mitophagy vs autophagy | ||
| مراجع | ||
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