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Culture Medium-dependent Antifungal Activity of Endophytic Deniquelata quercina against Sclerotinia sclerotiorum | ||
| Journal of Genetic Resources | ||
| دوره 12، شماره 2، 2026، صفحه 220-231 اصل مقاله (679.69 K) | ||
| نوع مقاله: Research Article | ||
| شناسه دیجیتال (DOI): 10.22080/jgr.2026.32810.1486 | ||
| نویسندگان | ||
| Aminallah Tahmasebi* 1؛ Somayeh Bakhshi2 | ||
| 1Department of Agriculture, Minab Higher Education Center, University of Hormozgan, Bandar Abbas, Iran | ||
| 2Department of Plant Protection, Faculty of Agriculture, Shiraz University, Shiraz, Iran | ||
| تاریخ دریافت: 13 خرداد 1405، تاریخ بازنگری: 18 شهریور 1405، تاریخ پذیرش: 13 تیر 1405 | ||
| چکیده | ||
| Sclerotinia sclerotiorum is a destructive soilborne phytopathogen affecting agricultural crops. Endophytic fungi as valuable antimicrobial sources provide sustainable biological control strategies. The type of culture medium substantially affects the antifungal activity of endophytic fungi. For each production medium, we used a corresponding assay medium with the same basal composition. This study evaluated four liquid culture media for their effects on the antifungal activity of Deniquelata quercina, an endophytic fungus isolated from the roots of Tamarix sp., against S. sclerotiorum. D. quercina isolate 8B2B was recovered from healthy Tamarix roots in Khormoj, Bushehr Province, Iran. The antifungal activity of D. quercina was assessed in four liquid media (potato dextrose broth, tryptic soy broth, malt extract broth, and yeast extract broth) using the minimum inhibitory concentration (MIC) and the percentage of mycelial growth inhibition on days 4 and 7, following a completely randomized design with three replicates. The ITS sequence of D. quercina isolate 8B2B was deposited in GenBank under accession number OP710154. The MIC results showed that culture filtrates of D. quercina obtained from potato dextrose broth and tryptic soy broth media exhibited the greatest inhibitory activity against S. sclerotiorum, with a MIC of 3.12%, compared with 6.25% for malt extract broth and >50% for yeast extract broth. Culture filtrates obtained from PDB and TSB media exhibited the highest inhibitory activity, with an MIC of 3.12%. In contrast, the filtrate obtained from malt extract broth (MEB) medium had an MIC of 6.25% and ranked next. The culture filtrate produced in yeast extract broth (YEB) medium showed no observable inhibitory effect on the growth of S. sclerotiorum up to a concentration of >50%. In the mycelial growth inhibition assay, potato dextrose broth showed the highest inhibition on day 7, with a mean of 99.60%, followed by tryptic soy broth at 94.05%. MEB and YEB demonstrated inhibition rates of 19.05% and 10.71%, respectively. To the best of our knowledge, this study provides the first evidence of antifungal activity of D. quercina against S. sclerotiorum. Moreover, our findings reveal a marked dependence of this activity on the culture medium, with potato dextrose broth yielding the most pronounced inhibitory effects. These results emphasize that optimization of fermentation conditions is essential for the reliable screening and development of endophytic fungi as potential candidates for further biocontrol evaluation. | ||
| کلیدواژه‌ها | ||
| Antifungal activity؛ Biological control؛ Culture medium؛ Endophytic fungus؛ Sclerotinia sclerotiorum | ||
| مراجع | ||
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Ait Assou, S., Anissi, J., Dufosse, L., & Fouillaud, M. (2025). Inducing and enhancing antimicrobial activity of mining-soil-derived actinomycetes through component modification of Bennett’s culture medium. Microbiology Research, 16(4), 72. https://doi.org/10.3390/microbiolres16040072 Alidadi, A., Kowsari, M., Javan-Nikkhah, M., Karami, S., Ariyawansa, H. A., & Jouzani, G. S. (2019). Deniquelata quercina sp. nov.; a new endophyte species from Persian oak in Iran. Phytotaxa, 405(4), 187-194. https://doi.org/10.11646/phytotaxa.405.4.2 Arianmanesh, R., Mehregan, I., Assadi, M., & Nejadsattari, T. (2016). Comparative morphology of the genus Tamarix (Tamaricaceae) in Iran. International Letters of Natural Sciences, 60, 1-12. https://doi.org/10.56431/p-6s8gxp Bakhshi, S., Eshghi, S., & Banihashemi, Z. (2023). Application of candidate endophytic fungi isolated from extreme desert adapted trees to mitigate the adverse effects of drought stress on maize (Zea mays L.). Plant Physiology and Biochemistry, 202, 107961. https://doi.org/10.1016/j.plaphy.2023.107961 Cao, F., Zhao, D., Chen, X.-Y., Liang, X.-D., Li, W., & Zhu, H.-J. (2017). Antifungal drimane sesquiterpenoids from a marine-derived Pleosporales sp. fungus. Chemistry of Natural Compounds, 53(6), 1189-1191. https://doi.org/10.1007/s10600-017-2236-4 Chen, X., Zhu, X., Ding, Y., & Shen, Y. (2011). Antifungal activity of tautomycin and related compounds against Sclerotinia sclerotiorum. The Journal of Antibiotics, 64(8), 563-569. https://doi.org/10.1038/ja.2011.55 Eo, J. K., Choi, J. W., & Eom, A. H. (2022). Diversity, distribution, and host plant of endophytic fungi: A focus on Korea. Mycobiology, 50(6), 399-407. https://doi.org/10.1080/12298093.2022.2154044 Gupta, A., Meshram, V., Gupta, M., Goyal, S., Qureshi, K. A., Jaremko, M., & Shukla, K. K. (2023). Fungal endophytes: Microfactories of novel bioactive compounds with therapeutic interventions; a comprehensive review on the biotechnological developments in the field of fungal endophytic biology over the last decade. Biomolecules, 13(7), 1038. https://doi.org/10.3390/biom13071038 Jha, P., Kaur, T., Chhabra, I., Panja, A., Paul, S., Kumar, V., & Malik, T. (2023). Endophytic fungi: Hidden treasure chest of antimicrobial metabolites interrelationship of endophytes and metabolites. Frontiers in Microbiology, 14, 1227830. https://doi.org/10.3389/fmicb.2023.1227830 Lai, D., Mao, Z., Zhou, Z., Zhao, S., Xue, M., Dai, J., … & Li, D. (2020). New chlamydosporol derivatives from the endophytic fungus Pleosporales sp. Sigrf05 and their cytotoxic and antimicrobial activities. Scientific Reports, 10(1), 8193. https://doi.org/10.1038/s41598-020-65148-0 Long, R. W., D Antonio, C. M., Dudley, T. L., & Hultine, K. R. (2021). Variation in salinity tolerance and water use strategies in an introduced woody halophyte (Tamarix spp.). Journal of Ecology, 109(11), 3807-3817. https://doi.org/10.1111/1365-2745.13758 Majerčíková, K., Labuda, R., Jaunecker, G., & Javoreková, S. (2015). The glucose effect on lincomycin production by Streptomyces lincolnensis var. lincolnensis DSM 40 355 on synthetic media. Journal of Microbiology, Biotechnology and Food Sciences, 4(4), 306-309. https://doi.org/10.15414/jmbfs.2015.4.4.306-309 Ohrtman, M. K., Sher, A. A., & Lair, K. D. (2012). Quantifying soil salinity in areas invaded by Tamarix spp. Journal of Arid Environments, 85, 114-121. https://doi.org/10.1016/j.jaridenv.2012.04.011 O’Sullivan, C. A., Belt, K., & Thatcher, L. F. (2021). Tackling control of a cosmopolitan phytopathogen: Sclerotinia. Frontiers in Plant Science, 12, 707509. https://doi.org/10.3389/fpls.2021.707509 Rouhi-Moghaddam, E., Sargazy, E., & Gholamalizadeh, A. (2015). Ecological properties of Tamarix habitats in Sistan Plain, Iran. ECOPERSIA, 3(4), 1201-1211. https://dor.isc.ac/dor/20.1001.1.23222700.2015.3.4.7.7 Ruiz, B., Chávez, A., Forero, A., García-Huante, Y., Romero, A., Sánchez, M., … & Langley, E. (2010). Production of microbial secondary metabolites: Regulation by the carbon source. Critical Reviews in Microbiology, 36(2), 146-167. https://doi.org/10.3109/10408410903489576 Sánchez, S., Chávez, A., Forero, A., García-Huante, Y., Romero, A., Sánchez, M., … & Ruiz, B. (2010). Carbon source regulation of antibiotic production. The Journal of Antibiotics, 63(8), 442-459. https://doi.org/10.1038/ja.2010.78 Senanayake, I. C., Pem, D., Rathnayaka, A. R., Wijesinghe, S. N., Tibpromma, S., Wanasinghe, D. N., … & Xie, N. (2022). Predicting global numbers of teleomorphic ascomycetes. Fungal Diversity, 114(1), 237-278. https://doi.org/10.1007/s13225-022-00498-w Smolińska, U., & Kowalska, B. (2018). Biological control of the soil-borne fungal pathogen Sclerotinia sclerotiorum- a review. Journal of Plant Pathology, 100(1), 1-12. https://doi.org/10.1007/s42161-018-0023-0 Tahmasebi, A., Ganbaatar, B., Bakhshi, S., Yun, H., & Lee, C. W. (2025). Identification, purification, and biochemical characterization of glucose oxidase from the newly isolated Talaromyces pinophilus 47 K9, an endophytic fungus from Prosopis stephaniana. International Journal of Biological Macromolecules, 321(3), 146402. https://doi.org/10.1016/j.ijbiomac.2025.146402 White, T.J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols, 18(1), 315-322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1 Xia, J., Zhang, S., Guo, J., Rong, Q., & Zhang, G. (2015). Critical effects of gas exchange parameters in Tamarix chinensis Lour on soil water and its relevant environmental factors on a shell ridge island in China’s Yellow River Delta. Ecological Engineering, 76, 36-46. https://doi.org/10.1016/j.ecoleng.2014.04.001 | ||
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