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Correlation between Expression of hsa-miR-490-5p and NFAT5 in Peripheral Blood Mononuclear Cell Obtained from Breast Cancer Patients | ||
| Journal of Genetic Resources | ||
| مقاله 5، دوره 5، شماره 1، خرداد 2019، صفحه 31-37 اصل مقاله (272.73 K) | ||
| نوع مقاله: Research Article | ||
| شناسه دیجیتال (DOI): 10.22080/jgr.2019.16143.1130 | ||
| نویسندگان | ||
| Amirhassan Nikfarjam1؛ Majid Pornour* 2؛ Mojtaba Sohrabi1؛ Hajar Vaseghi2 | ||
| 1Department of Biology, Qom Branch, Islamic Azad University, Qom, Iran | ||
| 2Department of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran | ||
| تاریخ دریافت: 02 بهمن 1397، تاریخ بازنگری: 21 آذر 1397، تاریخ پذیرش: 10 اسفند 1397 | ||
| چکیده | ||
| Breast cancer is a complex genetic disease that has an average annual incidence of two million people and the second leading cause of death among women all over the world. Micro-RNAs are consistently reported to regulate gene expression in all cancers. The present study, the correlation between the expression of hsa-miR-490-5p and nuclear factor of activated T-cells 5 (NFAT5) in breast cancer were investigated. NFAT5 nuclear accumulation occurs regardless of Wnt/β-catenin activated signaling in a substantial portion of breast cancer. The analysis of prediction target and dual-luciferase reporter assays supported that hsa-miR-490-5p directly targeted NFAT5 and suppressed the expression of NFAT5. In a cross-sectional comparative study, peripheral blood samples were collected from 30 subjects with breast cancer and 30 healthy individuals as a control group. Total mRNA was extracted from peripheral blood mononuclear cells (PBMCs) and cDNA was synthesized to study the NFAT5 and hsa-miR-490-5p gene expression variations by real-time PCR. A significant decrease was observed in gene expression and sera concentration of NFAT5, hsa-miR-490-5p in PBMCs of breast cancer patients. The obtained results indicated that hsa-miR-490-5p acts as oncomir in serum by targeting NFAT5 direct (p<0.05). | ||
| کلیدواژه‌ها | ||
| hsa-miR-490-5p؛ NFAT5؛ Breast cancer؛ PBMCs | ||
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