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تاثیر لیگاسیون عصب نخاعی بر بیان ژنهای Tweakو Fn14 عضله EDL موشهای نر ویستار پس از یک دوره تمرین HIT | ||
پژوهشنامه فیزیولوژی ورزشی کاربردی | ||
مقاله 10، دوره 15، شماره 30، دی 1398، صفحه 161-174 اصل مقاله (864.36 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22080/jaep.2019.15644.1848 | ||
نویسندگان | ||
عبدالرضا کاظمی* 1؛ الهه جهانشاهی2 | ||
1دانشیار گروه علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه ولی عصر، رفسنجان، کرمان، ایران | ||
2گروه فیزیولوژی ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه آزاد اسلامی، کرمان، - مرکز تحقیقات فیزیولوژی، دانشگاه علوم پزشکی کرمان، کرمان، ایران | ||
تاریخ دریافت: 20 بهمن 1397، تاریخ بازنگری: 05 اردیبهشت 1398، تاریخ پذیرش: 11 تیر 1398 | ||
چکیده | ||
سابقه و هدف: مسیر /Fn14 Tweak پاسخهای فیزیولوژیکی مختلف شامل بقا سلول، تکثیر، تمایز، رگزایی، مهاجرت و آپوپتوز را تنظیم میکند. هدف پژوهش حاضر بررسی اثر لیگاسیون عصب نخاعی بر بیان ژنهای Tweakو Fn14 عضله EDL موشهای نر ویستار پس از تمرین HIT بود. مواد و روشها: بدین منظور، 24 موش نر ویستار در 2 گروه 12 تایی کنترل (C) و تمرین (HIT) قرار گرفتند. گروهتمرین بعد از یک هفته آشناسازی برنامه 4 هفته HIT را انجام دادند. سپس به طور تصادفی ساده به دو گروه 6 تایی تمرین (HIT) و گروه لیگاسیون عصب نخاعی (HIT-SNL) قرار گرفتند. همزمان گروه کنترل نیز به دو گروه 6 تایی کنترل (C) و لیگاسیون عصب نخاعی (C-SNL) تقسیم شدند. 4 هفته پس از SNL موشها قربانی و عضله EDL و نعلی استخراج و بیان ژنها به روش کمیسازی دادهها Real-time PCR اندازهگیری شد و با استفاده از آزمون تحلیل واریانس دو راهه دادهها مورد تجزیه و تحلیل قرار گرفت. یافتهها: نتایج نشان داد کاهش فعالیت بدنی به شکل SNL سبب افزایش معنیداری بیان ژنهای Tweakو Fn14 میشود (به ترتیب 0001/0=P و 002/0=P). همچنین انجام تمرین HIT قبل از SNL در گروه تمرین- SNL بهطور قابل ملاحظهای بیان ژنهای Tweak و Fn14 را نسبت به گروه کنترل-SNL کاهش میدهد (به ترتیب 0001/0=P و 001/0=P). علاوه بر این، یافتهها نشان میدهد SNL اثر معنیداری بر نسبت وزن عضله نعلی به طول درشتنی دارد (01/0=P). از طرف دیگر انجام تمرینات HIT قبل از SNL سبب افزایش معنیدار آن شد (03/0=P). نتیجهگیری: کاهش فعالیت بدنی به شکل لیگاسیون با افزایش بیان ژنهای Tweak و Fn14 همراه بود.از طرف دیگر تمرین تناوبی شدید در کاهش ژنهای آتروفیک موثر بوده است. با توجه به این یافتهها، انجام تمرینات HIT قبل از ابتلا به بی فعالیتی بتواند روند آتروفی عضلانی را کاهش دهد. | ||
کلیدواژهها | ||
لیگاسیون عصب نخاعی؛ ژن Tweak؛ ژن Fn14 و تمرین تناوبی شدید | ||
عنوان مقاله [English] | ||
Effect of Spinal Nerve Ligation on The Expression of Tweak and Fn14 Genes in EDL Mucsle of Wistar Rats After HIT Training | ||
نویسندگان [English] | ||
Abdolreza Kazemi1؛ Elahe Jahanshahi2 | ||
1Department of Physical Education and Sport Sciences, Faculty of Literature and Humanities, Vali-e-Asr University, Rafsanjan, Iran. | ||
2Department of Physical Education and Sport Science, Kerman Branch, Islamic Azad University, Kerman, Iran. & Physiology Research Center, Kerman University of Medical, Kerman, Iran | ||
چکیده [English] | ||
Background and Purpose: The Tweak/Fn14 pathway regulated various physiological responses including cell survival, proliferation, differentiation, angiogenesis, migration and apoptosis. The purpose of the present study was to investigate the effect of SNL on the expression of Tweak and Fn14 genes in the EDL muscle of Wistar rats after HIT training. Materials and methods: For this purpose, 24 male Wistar rats were divided into 2 groups of control (C) (n=12) and training (HIT) (n=12). After one-week familiarization, training group participate in four HIT training. Then, they were randomly assigned to HIT (n=6) and HIT-SNL (n=6) groups. At the same time, the control group was divided into two groups: control (C) (n=6) and SNL (C-SNL) (n=6). 4 weeks after the SNL, wistar rats were sacrificed then EDL and soleuse muscle exetracted.Then gene expressions of Tweak and Fn14 measured with Real time PCR technique. Data were analyzed using 2-way ANOVA. Results: The results showed that inactivity via SNL has a significant increased of the expression of Tweak and Fn14 genes (P=0.000and P=0.002, respectively). Also, performed before SNL reduced the expression of Tweak and Fn14 genes in SNL-T compared with SNL-C (P=0.0001 and P=0.001 respectively). In addition, the findings indicated that SNL had a significant effect on the weight of soleuse muscle mass/ tibia length (P=0.01). On the other hand, HIT training before SNL significantly increased ratio weight soleuse muscle mass/ tibia length (P=0.03). Conclusion: The decrease in activity by SNL is associated with increased in expression of Tweak and Fn14 genes. On the other hand, HIIT training have been effective in reducing atrophic genes. According to these findings, performing HIT training before inactivity can reduced the muscular atrophy process. | ||
کلیدواژهها [English] | ||
Spinal Nerve Ligation, Tweak gene, Fn14 gene, High Intensity Interval Training | ||
مراجع | ||
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