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Comparing of Effects of Hydro-alcoholic, Ethanolic, and Methanolic Extracts of the Frangula alnus: Chemical Composition, Antimicrobial, and Synergism | ||
| Journal of Genetic Resources | ||
| مقاله 3، دوره 6، شماره 1، 2020، صفحه 20-33 اصل مقاله (1.27 M) | ||
| نوع مقاله: Research Article | ||
| شناسه دیجیتال (DOI): 10.22080/jgr.2020.2538 | ||
| نویسندگان | ||
| Armin Nejabatdoust1؛ Hakimeh Baghaei Daemi2؛ Ali Salehzadeh* 1؛ Seyyedeh Cobra Azimi3، 4؛ Sahereh Darafkan1؛ Fereshteh Fallah Digsara1؛ Moslem Pourebrahim5؛ Ramin seighalani5 | ||
| 1Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran | ||
| 2State Key Laboratory of Development of Veterinary Diagnostic Products, Huazhong Agricultural University, Wuhan, Hubei, China | ||
| 3Department of Chemistry, Faculty of Science, University of Gilan, Rasht, Iran | ||
| 4Caspian Sea Basin Research Center, University of Gilan, Rasht, Iran | ||
| 5Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization, Rasht, Iran | ||
| تاریخ دریافت: 20 آذر 1398، تاریخ بازنگری: 23 دی 1398، تاریخ پذیرش: 22 دی 1398 | ||
| چکیده | ||
| This study was conducted to evaluate the effect of different extraction solvents, including hydro-alcoholic (H-A), ethanolic (Et-OH), and methanolic (Me-OH), on chemical composition and synergistic antibacterial activity of Frangula alnus extract against Staphylococcus aureus and Escherichia coli. The chemical composition of the F. alnus bark extract was evaluated using FTIR, UV-Vis spectroscopy, and GC/MS analysis. The synergistic antimicrobial effect of the extracts with Ciprofloxacin (CIP) and Erythromycin (ERY) was evaluated using minimum inhibitory concentrations, combined disc, and checkerboard titration method. F. alnus contained alkanes, alkenes, phenols, alcohols, esters, terpenes, fatty acid, tetrazole, halo-alkane, anthraquinone as well aromatic, and plasticizer compounds in the ethanolic, methanolic, and hydro-alcoholic extracts. Total phenolic compounds for ethanolic, hydro-alcoholic and methanolic extracts were recorded 110.92±0.01, 95.27±0.01 and 126.6±0.02 g/L Gallic acid, respectively. Et-OH and H-A extracts in both combined and synergistic forms significantly inhibited bacterial growth. Concerning the antibacterial activity of the extracts, it was assumed that the compounds present in the plant extracts would destroy the bacterial cell membranes and consequently cause leakage of intracellular contents. Therefore these antibiotics in combination with the extracts would be able to affect their target sites (CIP; DNA gyrase and ERY; 50S subunit) more effectively. According to the results, H-A, Me-OH, and Et-OH extract efficiently in the synergistic state with erythromycin and ciprofloxacin can be a promising candidate to be used against bacterial pathogens. | ||
| کلیدواژه‌ها | ||
| Hydro-alcoholic؛ Erythromycin؛ Chemical composition؛ Ciprofloxacin؛ Gallic acid | ||
| مراجع | ||
|
Amsden GW. 1996. Erythromycin, clarithromycin, and azithromycin: are the differences real? Clin Ther 18: 56-72.
Calo JR, Crandall PG, O'Bryan CA, Ricke SC. 2015. Essential oils as antimicrobials in food systems- A Review. Food Control 54: 111-119.
Campoli-Richards DM, Monk JP, Price A, Benfield P, Todd PA, Ward A. 1988. Ciprofloxacin A review of its antibacterial activity, pharmacokinetic properties and therapeutic use. Drugs 35: 373-447.
Cockerill F, CLSI. 2012. Methods for dilution antimicrobial susceptibility tests f or Bacteria that grow aerobically; approved Standard-ninth edition. CLSI document M07-A9, Wayne, PA.
Coppo E, Marchese A. 2014. Antibacterial activity of polyphenols. Cur Pharm Biotechnol 15: 380-390.
Cowan MM. 1999. Plant products as antimicrobial agents. Clinic Microbiol Rev 12: 564-582.
Cox SD, Mann CM, Markham JL, Bell HC, Gustafson JE, Warmington JR, Wyllie SG. 2000. A mode of antibacterial action of the essential oil of Melaleuca alternifolia (tea tree oil). J Appl Microbiol 88: 170-175.
Carson CF, Mee BJ, Riley TV. 2002. Mechanism of action of Melaleuca alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage and salt tolerance assays and electron microscopy. Antimicrob Agents Chemother 46: 1914-1920.
Chouhan S, Sharma K, Guleria S. 2017. Antimicrobial activity of some essential oils-present status and future perspectives. Medicines 8: 4(3). pii: E58. doi: 10.3390/medicines4030058.
Daglia M. 2012. Polyphenols as antimicrobial agents. Curr Opin Biotechnol 23: 174-181.
Dilika, F, Bremner PD, Meyer JJM. 2000. Antibacterial activity of linoleic and oleic acids isolated from Helichrysum pedunculatum: a plant used during circumcision rites. Fitoterapia 71: 450-452.
Daly SM, Elmore BO, Kavanaugh JS, Triplett KD, Figueroa M, Raja HA, El-Elimat T, Crosby HA, Femling JK, Cech NB, Horswill AR. 2015. ω-Hydroxyemodin limits Staphylococcus aureus quorum sensing-mediated pathogenesis and inflammation. Antimicrob Agents Chemother 59: 2223-2235
Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S and Ju, YH. 2014. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J Food Drug Anal 296-302.
Elisha IL, Botha FS, McGaw LJ and Eloff JN. 2017. The antibacterial activity of extracts of nine plant species with good activity against Escherichia coli against five other bacteria and cytotoxicity of extracts. BMC Complement Altern Med 17: 133. https://doi.org/10.1186/s12906-017-1645-z.
Farrar L J. 1995. Trees of the northern United States and Canada. Ames, Iowa: Blackwell Publishing.
Gleason HA, Cronquist A. 1991. Manual of vascular plants of northeastern United States and adjacent Canada. 2nd Ed. New York Botanical Garden, New York, USA.
Huang W, Wang JQ, Song HY, Zhang Q and Liu GF. 2017. Chemical analysis and in vitro antimicrobial effects and mechanism of action of Trachyspermum copticum essential oil against Escherichia coli. Asian Pac J Trop Med 10: 663-669.
Hutchings A, Cock IE. 2018. The interactive antimicrobial activity of Embelica officinalis gaertn. fruit extracts and conventional antibiotics against some bacterial triggers of autoimmune inflammatory diseases. Pharmacog J 10: 654-62.
Hamed MM, Refahy LA and Abdel-Aziz MS. 2015. Evaluation of antimicrobial activity of some compounds isolated from Rhamnus cathartica L. Orient J Chem 31: 1133-1140.
Jain PK, Soni A, Jain P and Bhawsar J. 2016. Phytochemical analysis of Mentha spicata plant extract using UV-VIS, FTIR and GC/MS technique. J Chem Pharm Res 8(2): 1-6.
Kremer D, Kosalec I, Locatelli M, Epifano F, Genovese S, Carlucci G and Končić MZ. 2012. Anthraquinone profiles, antioxidant and antimicrobial properties of Frangula rupestris (Scop.) Schur and Frangula alnus Mill. Bark. Food Chem 131: 1174-1180.
Kanoh S, Rubin BK. 2010. Mechanisms of action and clinical application of macrolides as immunomodulatory medications. Clin. Microbiol 23: 590-615.
Kalaichelvi K, Dhivya SM. 2017. Screening of phytoconstituents, UV-vis spectrum and FTIR analysis of Micrococca mercurialis (L.) Benth. Int J Herb 5(6): 40-44.
Karpagasundari C, Kulothungan S. 2014 Analysis of bioactive compounds in Physalis minima leaves using GC MS, HPLC, UV-vis and FTIR techniques. J Pharmacogn Phytochem 3: 196-201.
Miao J, Liu G, Ke C, Fan W, Li C, Chen Y, Dixon W, Song M, Cao Y and Xiao H. 2016. Inhibitory effects of a novel antimicrobial peptide from kefir against Escherichia coli. Food Control 65: 63-72.
Mukherjee PK, Saritha GS and Suresh B. 2002. Antimicrobial potential of two different Hypericum species available in India. Phytother Res 16: 692-695.
Malmir M, Ferreira E, Serrano R, Gomes ET, Canica M and Silva O. 2015. In vitro anti-Neisseria gonorrhoeae activity of Senna podocarpa root extracts. Indian Crop Prod 76: 467-471.
Muthuselvi C, Pandiarajan SS, Ravikumar B, Athimoolam S, Krishnakumar RV. 2018. Halogen substituted indeno quinoxaline derivative crystal: a spectroscopic approach. Asian J Applied Sci 11: 29-37.
Nejabatdoust A, Salehzadeh A, Zamani H, Moradi-Shoeili Z. 2019. Synthesis, characterization and functionalization of ZnO nanoparticles by glutamic acid (Glu) and conjugation of ZnO@Glu by thiosemicarbazide and its synergistic activity with ciprofloxacin against multi-drug resistant Staphylococcus aureus. J Clust Sci 30: 329-336
Nascimento GGF, Locatelli J, Freitas PC. 2000. Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic resistant bacteria. Braz J Microbiol 31: 247-256.
Nallasamy P, Anbarasan PM and Mohan S. 2002. Vibrational spectra and assignments of cis-and trans-1, 4-polybutadiene. Turkish J Chem 26: 105-112.
Ordonez AAL, Gomez JD and Vattuone MA. 2006. Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food Chem 97: 452-458.
Orhan G, Bayram A, Zer Y and Balci I. 2005. Synergy tests by E test and checkerboard methods of antimicrobial combinations against Brucella melitensis. J Clin Microbiol 43: 140-143.
Oliveira RN, Mancini MC, Oliveira FCSD, Passos TM, Quilty B, Thiré RMDSM, McGuinness GB. 2016 FTIR analysis and quantification of phenols and flavonoids of five commercially available plants extracts used in wound healing. Matéria. 21: 767-779.
Roudbaraki SJ, Nori-Shargh D. 2016. Analysis of the volatile constituents of Frangula alnus Mill. from Iran. Russ Chem Bull 65: 2770-2772
Radulovic NS, Blagojevic PD, Stojanovic-Radic ZZ, Stojanovic NM. 2013. Antimicrobial plant metabolites: structural diversity and mechanism of action. Curr Med Chem 20: 932-952.
Rad-Moghadam K, Azimi SC. 2012. Synthesis of novel oxindolylpyrrolo [2, 3-d] pyrimidines via a three-component sequential tandem reaction. Tetrahedron 68 (47): 9706-9712.
Salehzadeh A, Doulabi H, Sadat M, Sohrabnia B, Jalali A. 2018. The effect of thyme (Thymus vulgaris) extract on the expression of norA efflux pump gene in clinical strains of Staphylococcus aureus. J Genet Res 4(1): 26-36.
Souza EL, de Barros JC, de Oliveira CEV, da Conceição ML. 2010. Influence of Origanum vulgare L. essential oil on enterotoxin production, membrane permeability and surface characteristics of Staphylococcus aureus. Int J Food Microbiol 137: 308-311.
Stenz L, Franc¸ois P, Fischer A, Huyghe A, Tangomo M, Hernandez D, Cassat J, Linder P, Schrenzel, J. 2008. Impact of oleic acid (cis -9-octadecenoic acid) on bacterial viability and biofilm production in Staphylococcus aureus. FEMS Microbiol Lett 287: 149-155.
Sandosh TA, John Peter MP, Raj JY. 2013. Phytochemical analysis of Stylosanthes fruticosa using UV-vis, FTIR and GC-MS. Res J Chem Sci 3: 14-23.
Tchakam PD, Lunga PK, Kowa TK, Lonfouo AH, Wabo HK, Tapondjou LA, Tane P, Kuiate JR. 2012. Antimicrobial and antioxidant activities of the extracts and compounds from the leaves of Psorospermum aurantiacum Engl. and Hypericum lanceolatum Lam. BMC Complement Altern Med 12: 136. Doi: 10.1186/1472-6882-12-136.
Tenson T, Lovmar M, Ehrenberg M. 2003. The mechanism of action of macrolides, lincosamides and streptogramin B reveals the nascent peptide exit path in the ribosome. J Mol Biol 25: 1005-14.
Ultee A, Smid EJ. 2001. Influence of carvacrol on growth and toxin production by Bacillus cereus. Int J Food Microbiol 64: 373-378.
Verma P. 2007. Methods for determining bactericidal activity and antimicrobial interactions: synergy testing, time-kill curves, and population analysis. In: Schwalbe R, Steele-Moore L, Goodwin AC, eds. Antimicrobial susceptibility testing protocols. Roca Raton, Florida, USA: CRC Press. 275-298. | ||
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