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Expression of Survivin in the Formalin-Fixed, Paraffin-embedded Specimens of Human Osteosarcoma May Be Used as a Diagnostic Marker | ||
| Journal of Genetic Resources | ||
| مقاله 6، دوره 6، شماره 1، 2020، صفحه 49-53 اصل مقاله (662.29 K) | ||
| نوع مقاله: Research Article | ||
| شناسه دیجیتال (DOI): 10.22080/jgr.2020.18366.1175 | ||
| نویسندگان | ||
| Esmaeil Babaei* 1؛ Bashdar Mahmud Hussen2؛ Maryam Abedi1؛ Hewa Jalal Azeez1 | ||
| 1Department of Animal Biology, School of Natural Sciences, University of Tabriz, Tabriz-Iran | ||
| 2College of Pharmacy, Hawler Medical University, Erbil, Iraq | ||
| تاریخ دریافت: 27 آذر 1398، تاریخ بازنگری: 29 دی 1398، تاریخ پذیرش: 23 فروردین 1399 | ||
| چکیده | ||
| Osteosarcoma is the most frequent malignant bone tumor with a peak incidence in the second and third decades of life. Diagnosis of patients with osteosarcoma is limited to clinico-pathological parameters whereas molecular markers of tumor initiation and promotion have not yet been identified. We aimed to study the expression of the anti-apoptotic survivin gene in osteosarcoma specimens. A total of 35 formalin-fixed, paraffin-embedded (FFPE) specimens including 25 tumoral and 20 non-tumoral paired-margins were collected. The expression of survivin was evaluated by employing real-time PCR and immunohistochemistry. Our data showed that survivin is differently expressed in osteosarcoma samples rather than non-tumoral margins. Real-time PCR showed that survivin is up-regulated in osteosarcoma specimens compared to margins (p <0.01). Furthermore, IHC confirmed that the survivin protein is significantly found in malignant parts of paraffin blocks. Our data show that survivin expression is modulated in osteosarcoma and may be considered as a diagnostic marker for further studies. Our data showed that survivin is differently expressed in osteosarcoma samples rather than non-tumoral margins. Real-time PCR showed that survivin is up-regulated in osteosarcoma specimens compared to margins (p <0.01). Furthermore, IHC confirmed that the survivin protein is significantly found in malignant parts of paraffin blocks. Our data show that survivin expression is modulated in osteosarcoma and could be considered as a diagnostic marker for further studies. | ||
| کلیدواژه‌ها | ||
| Tumor marker؛ Real-time PCR؛ FFPE blocks؛ Immunohistochemistry | ||
| مراجع | ||
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