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تأثیر هیستومورفومتریک و هیستولوژیک شنای استقامتی بر بیضهی موشهای بالغ نر ویستار | ||
پژوهشنامه فیزیولوژی ورزشی کاربردی | ||
مقاله 4، دوره 16، شماره 31، شهریور 1399، صفحه 59-70 اصل مقاله (971.05 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22080/jaep.2020.17890.1922 | ||
نویسندگان | ||
مهدی اسدی1؛ محمد رحمانی* 2؛ اسمعیل نصیری3؛ علی کلانتری حصاری4؛ میلاد خسروی صدر1؛ مهدی عزتی فر1 | ||
1دانشجوی کارشناسی ارشد فیزیولوژی ورزشی، گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه شاهد، تهران، ایران | ||
2استادیار گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه شاهد، تهران، ایران. | ||
3استادیار گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه شاهد، تهران، ایران | ||
4گروه پاتوبیولوژی، دانشکده پیرادامپزشکی، دانشگاه بوعلی سینا، همدان، ایران. | ||
تاریخ دریافت: 29 دی 1398، تاریخ بازنگری: 09 اردیبهشت 1399، تاریخ پذیرش: 03 تیر 1399 | ||
چکیده | ||
سابقه و هدف: فعالیت بدنی در کنار اثر مطلوب بر برخی سیستمهای بدنی ممکن است با اختلال برخی دیگر همراه باشد. با وجود اینکه تمرین استقامتی «تمرین طلایی» برای سلامت عمومی به شمار میرود؛ اما براساس نتایج مطالعاتی این نوع تمرین میتواند با اختلال سیستم تولید مثل همراه باشد؛ هدف از مطالعهی حاضر بررسی تأثیر شنای استقامتی بر شاخصهای هیستومورفومتریک بیضه موش صحرایی نر بالغ ویستار (موش) بود. مواد و روشها: برای این منظور 10 موش، تصادفی به 2 گروه شاهد (5=n) و شنای استقامتی (5=n) تقسیم شدند. موشهای گروه آزمایش 6 هفته، هفتهای سه جلسه تحت شنای استقامتی 45 دقیقهای قرار گرفتند. 48 ساعت پس از آخرین جلسه شنا، موشها به روش آسانکشی قربانی و با فتها برای بررسی پارامترهای هیستومورفومتریک به آزمایشگاه ارسال شد. دادهها با آزمون t مستقل در سطح آلفای 05/0 در محیط نرم افزار SPSS نسخهی 24 تحلیل شد. یافته ها: شاخصهای تمایز لولهای(TDI) (001/0=p )، ضریب تجمعی (RI)(00/0=p)، شاخص اسپرمیوژنز(SI)(00/0=p )، تعداد سلولهای سرتولی (01/0=p ) گروه تمرین در مقایسه با گروه کنترل بهطور معنی داری کمتر بود؛ اما تفاوت از نظر شاخصهای سلولهای لیدیگ (45/0=p)، ضخامت کپسول بیضه (07/0=p)، قطر لولهی اسپرمساز(92/0=p)، ارتفاع اپیتلیوم لولهی اسپرمساز(117/0=p)، تعداد لولههای اسپرمساز (51/0=p ) و قطر لومن داخلی لولههای اسپرمساز (29/0=p ) معنیدار نبود. نمای هیستولوژیکی بیضه گروه شنا حاکی از اختلال شدید اسپرماتوژنز بود. نتیجهگیری: بنابراین، شنای استقامتی با پروتکل استفادهشده در این پژوهش میتواند زمینهساز اختلال سیستم تولید مثل و باروری باشد. با توجه به جایگاه فعالیت استقامتی در سلامت عمومی به ویژه سلامت قلبی-عروقی، برای پیشگیری از پیشامد چنین عارضهای باید به فکر راهکارهای مکمل یا تغییر مؤلفههای تمرینی برای فعالیت استقامتی بود. | ||
کلیدواژهها | ||
شنای استقامتی؛ سیستم تولید مثل؛ ناباروی؛ هیستومورفومتری | ||
عنوان مقاله [English] | ||
Histomorphometric and Histologic Effect of Endurance Swimming on the Testis of Adult Wistar Rats | ||
نویسندگان [English] | ||
Mehdi Asadi1؛ Mohammad Rahmani2؛ Esmail Nasiri3؛ Ali Kalantari- Hesari4؛ Milad Khosravi -Sadr1؛ Mehdi Ezzatifar1 | ||
1Department of Sports Science, Faculty of Humanities, Shahed University, Tehran, Iran | ||
2Department of Sports Science, Faculty of Humanities, Shahed University, Tehran, Iran | ||
3Department of Sports Science, Faculty of Humanities, Shahed University, Tehran, Iran | ||
4Department of Pathobiology, Faculty of Veterinary Sciences, Bu-Ali Sina University, Hamedan, Iran. | ||
چکیده [English] | ||
Background Purpose: Exercise along with the desired effect on some physical systems may be associated with the disorder of other systems. Although endurance training is a "golden exercise" for general health, but based on the scientific studies, it can be associated with reproductive system disorders; the purpose of the present study was to investigate the effects of endurance swimming on the histomorphometric indices of testis in adult male Wistar rats. Methodology: For this purpose, rats (N=10) were randomly divided into control (C) (n=5) and endurance swimming (ES) (n=5) groups. The rats in ES group were exposed to 45 minutes' endurance swimming, three sessions a week for 6 weeks. Forty-five hours after the last swim session, rats were euthanized, research ethics considered. Their testes were placed in 10% buffered formalin solution and sent to the laboratory for histomorphometric evaluations. Data were analysed using independent t-test at α=0.05. Results: Tubular differentiation indices (TDI) (p=0.001), cumulative coefficient (RI) (p=0.00), spermiogenesis index (SI) (p=0.00) and number of Sertoli cells (p=0.01) of ES group were significantly less than C group, but differences in Leydig cell parameters (p=0.45), testicular capsule thickness (p=0.07), tube diameter Spermatogenesis (p=0.92), sperm tube-forming epithelium height (p=0.117), number of spermatogenic tubes (p=0.51) and inner tube lumen diameter Spermatogenesis (p=0.29) were not significant. The histological profile of the testis of ES group showed severe spermatogenesis. Conclusion: Endurance swimming protocol of this study can provoke reproductive and infertility disorders, and given the role of endurance activity in public health, especially cardiovascular health, complementary strategies should be considered to prevent the occurrence of such complications. | ||
کلیدواژهها [English] | ||
Endurance Swimming, Reproductive System, Infertility, Histomorphometric | ||
مراجع | ||
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