|تعداد مشاهده مقاله||6,234,374|
|تعداد دریافت فایل اصل مقاله||4,571,727|
|Journal of Genetic Resources|
|دوره 9، شماره 1، اردیبهشت 2023، صفحه 11-16 اصل مقاله (332.63 K)|
|نوع مقاله: Research Article|
|شناسه دیجیتال (DOI): 10.22080/jgr.2022.24104.1326|
|Zahra Hashem Matori1؛ Mahyar Gerami* 1؛ Kamran Ghaedi2؛ Parastoo Majidian* 3|
|1Biology Department, Sana Institute of Higher Education, Sari, Iran|
|2Biology Department, Isfahan University, Isfahan, Iran|
|3Crop and Horticultural Science Research Department, Mazandaran Agricultural and Natural Resources Research and Education Center, AREEO, Sari, Iran|
|تاریخ دریافت: 21 مرداد 1401، تاریخ بازنگری: 17 مهر 1401، تاریخ پذیرش: 03 مهر 1401|
|The main storage form of phosphorus is phytate (Myo-inositol Hexakisphosphate) in legume crops, such as clover and alfalfa, which are of high importance in terms of nutrition for humans and animals. In animals, due to a lack of phytase enzymes in their intestines, it is not possible to break the phytic acid (a nutritional constituent). Hence, phytic acid acts as an anti-nutritional chelating agent for various metal ions like Ca, Mg, Fe, Zn, etc., so it reduces the nutritive quality of food. Since phytase is an important enzyme in the food/feed industry, the objective of this study is to isolate phytase-producing bacteria cells to analyze phytate molecules. The present study was conducted in the laboratory of the Sana Institute of Higher Education. In this study, 8 soil samples of alfalfa and clover fields located in Isfahan (Khomeini Shahr and Morche Khort Regions) were collected and several bacteria isolates were separated using differential media. To examine the phytase activity, the isolated bacteria on the specific media fortified with phytate were cultivated and positive phytate bacteria were identified using morphological traits, biochemical tests, and 16srRNA sequencing determination. The data obtained from quantitative properties showed that two isolates of B1 and D1 have 17 and 20 mm size of zone diameters, respectively. Based on morphological properties, the B1 bacteria showed a big size of the colony, with a bump hanging in the margin surrounding the colony and white pigment, which was gram-positive. However, the D1 sample indicated a small colony size, with a wavy margin, smooth bump, and creamy pigment which was gram-positive. By biochemical recognition test, among all bacteria cells, two bacteria colonies were distinguished concerning the phytase activity and were recognized as Bacillus sp. In addition, the 16srRNA sequencing analysis showed that one strain belongs to Bacillus paralicheniformis (95%) and the other one is related to Bacillus endophyticus (95%), both of which are found in soil usually.|
|16srRNA؛ Bacillus؛ Gene؛ Phytase؛ Phytic acid|
Borgi MA, Boudebbouze S, Aghajari N, Szukala F, Pons N, Maguin E, …, Rhimi M. 2014. The attractive recombinant phytase from Bacillus licheniformis: biochemical and molecular characterization. Appl Microbiol Biotechnol 98(13): 5937-5947.
Chen A, Liu X, Cui C, Yang C, Wang Y, Bu X, …, Yang Y. 2019. An evaluation of phytase for Channel catfish (Ictalurus punctatus) fed all plant‐protein diet: Growth performance, nutrient utilization and P equivalency value. Aqua Nut 25(1): 215-224.
Choi TY, Dooley KJ, Rungtusanatham M. 2001. Supply networks and complex adaptive systems: control versus emergence. J Oper Man 19(3): 351-366.
Dev SS, Nisha EA, Venu A. 2016. Biochemical and molecular characterization of efficient phytase producing bacterial isolates from soil samples. Int J Curr Microbiol Applied Sci 5(5): 218-226.
Gupta RK, Gangoliya SS, Singh NK. 2015. Reduction of phytic acid and enhancement of bioavailable micronutrients in food grains. J Food Sci Technol 52(2): 676-684.
Haefner S, Knietsch A, Scholten E, Braun J, Lohscheidt M, Zelder O. 2005. Biotechnological production and applications of phytases. Appl Microbiol Biotechnol 68(5): 588-597.
Haefner S, Knietsch A, Scholten E, Braun J, Lohscheidt M, Zelder O. 2005. Biotechnological production and applications of phytases. Appl Microb Biotechnol 68(5): 588-597.
Hosseinkhani H, Hosseinkhani M. 2009. Biodegradable polymer-metal complexes for gene and drug delivery. Curr Drug Safety 4(1): 79-83.
Koinetzny U, Greiner R. 2004. Bacterial phytase: potential application, in vivo function and regulation of its synthesis. Brazilian J Microbiol 35: 12-18.
Kumar Singh N, Kumar Joshi D, Kishor Gupta R. 2013. Isolation of phytase producing bacteria and optimization of phytase production parameters. Jundishapur J Microbiol 6(5): 1-6.
Mittal A. Singh G. Goyal V. Yadav A. Aneja KR. Gautam SK. Aggarwal NK. 2011. Isolation and biochemical characterization of acido-thermophilic extracellular phytase producing bacterial strain for potential application in poultry feed. Jundishapur J Microbiol 4(4): 273- 282.
Naseri F, Bahador N, Baserisalehi M, Kargar M. 2015. Isolating tricalcium phosphate solubilizing bacteria from wheat and oat soil in Marvdasht. J Microb World 8(22): 38-46.
Nazemi A, Gholamian S, Miri Nargesi M. 2013. Isolation and molecular identification of Bacillus species that produce extracellular L asparaginase, an anti-cancer enzyme, from soils. New Cell Mol Biotech J 3(11): 9-13.
Parhamfar M, Abtahi H, Parhamfar M. 2017. Optimization of culture conditions for the production of phytase enzyme by Bacillus subtilis soil isolates. J Microb World 9(4): 315-325.
Raghothama KG, Karthikeyan AS. 2005. Phosphate acquisition. Plant Soil 274(1): 37-49.
Ranjan P, Das MP, Kumar MS, Anbarasi P, Sindhu S, Sagadevan E, …, Arumugam P. 2013. Green synthesis and characterization of silver nanoparticles from Nigella sativa and its application against UTI causing bacteria. J Acad Ind Res 2(1): 45-49.
Rodriguez H, Fraga R. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 17: 319-339.
Sarikhani MR, Chalabianlu N, Alavikia SS. 2016. Distribution of phosphate solubilizing bacteria and soil phosphate activity in different land uses. J Water Soil 29(6): 1662-1673.
Shieh TR, Ware JH. 1968. Survey of microorganisms for the production of extracellular phytase. Appl Microbiol 16(9): 1348-1351.
Teimouri M, Korori SAA, Matinizadeh M, Khoshnevis M. 2004. Isolation and identification of phosphate solubilizing bacteria from Vaz forest soil. Pajouh Sazand 17(4): 57-64.
Teimouri M, Sadegzadeh Hallaj MH, Alizadeh T, Matinizadeh M. 2020. The effect of phosphate solubilizing bacteria on growth and nutritional status of oak seedlings. J Plant Res 33(3): 689-704.
Whitelaw MA. 2000. Growth promotion of plants inoculated with phosphate-solubilizing fungi. In: Whitelaw MA. Adv Agron 69: 99-151.
تعداد مشاهده مقاله: 147
تعداد دریافت فایل اصل مقاله: 258