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Investigating the Effect of Thymol Active Ingredient and ZnO Nanoparticle Conjugated by Thiosemicarbazone on the Expression of Efflux Pump and Biofilm Genes in Pseudomonas aeruginosa | ||
Journal of Genetic Resources | ||
دوره 9، شماره 1، 2023، صفحه 75-82 اصل مقاله (635.77 K) | ||
نوع مقاله: Research Article | ||
شناسه دیجیتال (DOI): 10.22080/jgr.2023.24604.1338 | ||
نویسندگان | ||
Hadiseh Mokhtari؛ Seyedeh Tooba Shafighi* ؛ Ali Salehzadeh؛ Somayeh Ataei jaliseh | ||
Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran | ||
تاریخ دریافت: 02 مهر 1401، تاریخ بازنگری: 06 بهمن 1401، تاریخ پذیرش: 10 آبان 1402 | ||
چکیده | ||
Finding novel antimicrobials to treat drug-resistant Psudomonas aeruginosa is a major health challenge. In this study, ZnO nanoparticles functionalized by Thiosemicarbazone nanoparticles (ZnO@Glu-TSC NPs) were synthesized and the effect of the NPs alone and in combination with thymol on the expression of biofilm and efflux pump genes in P. aeruginosa was investigated. Physicochemical features of the ZnO@Glu-TSC NPs were evaluated by FT-IR, XRD, EDS-mapping, and SEM and TEM imaging. The inhibitory effect of ZnO@Glu-TSC NPs and thymol, alone and in combination, were determined by broth microdilution method, and quantitative PCR was used to evaluate the expression of the pelA, pslA, algD, mexA, mexB, and mexX genes. The synthesized NPs were almost spherical, without impurities, and in a size range of 20 to 60 nm. Simultaneous treatment of P. aeruginosa with ZnO@Glu-TSC and thymol had a significantly stronger inhibitory effect (MIC: 3.12-25.5 µg/mL) than either agent alone. The relative expression of the pelA, pslA, and algD genes in P. aeruginosa strains treated with ZnO@Glu-TSC+thymol was reduced by 0.44, 0.43, and 0.46 folds, respectively. Furthermore, the expression of mexA, mexB, and mexX genes decreased in P. aeruginosa strains treated with ZnO@Glu-TSC+thymol (0.42, 0.45, and 0.41 folds, respectively). Also, it was found that the combination of ZnO@Glu-TSC and thymol could synergically reduce the expression of the mentioned genes in comparison with either agent alone. This study showed that ZnO@Glu-TSC NPs and thymol synergically inhibited biofilm maturation and efflux pump systems in P. aeruginosa strains and could be considered a novel antibacterial candidate against P. aeruginosa strains. | ||
کلیدواژهها | ||
Biofilm؛ Efflux pump؛ Quantitative PCR؛ Thiosemicarbazone؛ Zinc oxide | ||
مراجع | ||
Atshan SS, Shamsudin MN, Karunanidhi A., van Belkum A, Lung LTT, Sekawi Z, …, Abduljaleel SA, 2013. Quantitative PCR analysis of genes expressed during biofilm development of methicillin resistant Staphylococcus aureus (MRSA). Infect Genet Evol 18: 106-112.
Tuon FF, Dantas LR, Suss PH, Tasca Ribeiro VS. 2022. Pathogenesis of the Pseudomonas aeruginosa Biofilm: A Review. Pathogens 11(3): 300. https://doi.org/10.3390/pathogens 1103 0300.
Dai L, Wu TQ, Xiong YS, Ni HB, Ding Y, Zhang WC, ..., Yu J. 2019. Ibuprofen-mediated potential Inhibition of biofilm development and quorum sensing in Pseudomonas aeruginosa. Life Sci 237: 116947. https:// doi. org/ 10. 1016/j. lfs. 2019. 116947.
Ha DG, O'Toole GA. 2015. C-di-GMP and its effects on biofilm formation and dispersion: a Pseudomonas aeruginosa review. Microbiol Spectr 3(2). https:// doi. org/ 10.1128/ microbiolspec. MB- 0003-2014.
Franklin MJ, Nivens DE, Weadge JT, Howell PL. 2011. Biosynthesis of the Pseudomonas aeruginosa extracellular polysaccharides, alginate, Pel, and Psl. Front Microbiol 2: 167. https:// doi. org/10.3389/fmicb. 2011. 00167.
Ma L, Conover M, Lu H, Parsek MR, Bayles K, Wozniak DJ. 2009. Assembly and development of the Pseudomonas aeruginosa biofilm matrix. PLoS Pathog 5(3): e1000354. https:// doi. org/10.1371/ journal. ppat.1000354
Coulon C, Vinogradov E, Filloux A, Sadovskaya I. 2010. Chemical analysis of cellular and extracellular carbohydrates of a biofilm-forming strain Pseudomonas aeruginosa PA14. PLoS One 5(12): e14220. https:// doi. org/10.1371/journal.pone.0014220.
Dreier J, Ruggerone P. 2015. Interaction of antibacterial compounds with RND efflux pumps in Pseudomonas aeruginosa. Front Microbiol 6: 660. https:// doi. org/10.3389/fmicb. 2015.00660.
Kazemi-Pasarvi S, Ebrahimi NG, Shahrampour D, Arab-Bafrani Z. 2020. Reducing cytotoxicity of poly (lactic acid) – based / zinc oxide nanocomposites while boosting their antibacterial activities by thymol for biomedical applications. Int J Biol Macromol 164: 4556-4565.
Najafloo R, Behyari M, Imani R, Nour S. 2020. A mini-review of Thymol incorporated materials: Applications in antibacterial wound dressing. J Drug Deliv Sci Technol 60: 101904. https://doi. org/ 10.1016/ j.jddst. 2020. 101904
Nejabatdoust A, Zamani H, Salehzadeh A. 2019. Functionalization of ZnO nanoparticles by glutamic acid and conjugation with thiosemicarbazide alters expression of efflux pump genes in multiple drug-resistant Staphylococcus aureus strains. Microb Drug Resist 25(7): 966-974.
Pfaffl MW. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29: e45. https://doi.org/10.1093/nar/29.9.e45.
Foo KL, Hashim U, Muhammad K, Voon CH. 2014. Sol–gel synthesized zinc oxide nanorods and their structural and optical investigation for optoelectronic application. Nanoscale Res Lett 9(1): 1-10.
Khan MF, Ansari AH, Hameedullah M, Ahmad E, Husain FM, Zia Q, ..., Aliev G. 2016. Sol-gel synthesis of thorn-like ZnO nanoparticles endorsing mechanical stirring effect and their antimicrobial activities: Potential role as nano-antibiotics. Sci Rep 6(1): 1-12.
Sirelkhatim A, Mahmud S, Seeni A, Kaus NHM, Ann LC, Bakhori SKM, ..., Mohamad D. 2015. Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism. Nanomicro Lett 7(3): 219-242.
Li M, Zhu L, Lin D. 2011. Toxicity of ZnO nanoparticles to Escherichia coli: mechanism and the influence of medium components. Environ Sci Technol 45(5): 1977-1983.
Tada R, Chavda N, Shah MK. 2011. Synthesis and characterization of some new thiosemicarbazide derivatives and their transition metal complexes. J Chem Pharm Res 3(2): 290-297.
Yanishlieva NV, Marinova EM, Gordon MH, Raneva VG. 1999. Antioxidant activity and mechanism of action of thymol and carvacrol in two lipid systems. Food Chem 64(1): 59-66.
Askoura M, Mottawea W, Abujamel T, Taher I. 2011. Efflux pump inhibitors (EPIs) as new antimicrobial agents against Pseudomonas aeruginosa. Libyan J Med 6: 5870. doi: 10.3402/ ljm.v6i0. 5870. | ||
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