آمار
| تعداد نشریات | 28 |
| تعداد شماره‌ها | 426 |
| تعداد مقالات | 4,164 |
| تعداد مشاهده مقاله | 6,739,395 |
| تعداد دریافت فایل اصل مقاله | 4,988,827 |
Molecular Identification of Residual DNA Separated from the Persian Sturgeon (Acipenser persicus) for Modeling eDNA Evaluation in Aquatic Ecosystem | ||
| Journal of Genetic Resources | ||
| دوره 9، شماره 1، اردیبهشت 2023، صفحه 103-110 اصل مقاله (530.75 K) | ||
| نوع مقاله: Research Article | ||
| شناسه دیجیتال (DOI): 10.22080/jgr.2023.25221.1348 | ||
| نویسندگان | ||
| Hor Torabi Jafroudi1؛ Shirin Jamshidi* 2؛ Soheila Talesh Sasani1؛ Ali Bani1 | ||
| 1Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran | ||
| 2International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran | ||
| تاریخ دریافت: 04 مهر 1401، تاریخ بازنگری: 04 اردیبهشت 1402، تاریخ پذیرش: 13 آبان 1401 | ||
| چکیده | ||
| Considering the importance of Caspian Sea sturgeon conservation according to CITES rules, finding effective and efficient methods for tracing and identifying sturgeon species are necessary. Residual DNA detection in fish ponds can be used as a model for tracing fish environmental DNA (eDNA) in rivers and seas. This method of DNA detection is non-invasive and advantageous for the conservation of critically endangered sturgeons. Sampling was done from the water of the Persian sturgeon fish pond and fixed with precipitation premix solution (ethanol and acetate sodium). DNA was extracted from fixed water, and fine tissue of two sturgeon species in the Caspian Sea, Sterlet, and Siberian sturgeon. The positive specificity of primers was checked by conventional PCR for sturgeons with fine tissue DNA samples. The linear relationship between the threshold cycle (ct) value and the Persian Sturgeon DNA concentration was measured by the Mini-Barcoding quantitative real-time PCR. DNA samples of fish ponds generated a curve that could not be produced in negative control and irrelevant (non-sturgeon) genomic DNA. Although residual DNA of Persian sturgeons was detected in fish pond water at picogram level through the quantitative method, molecular diagnosis with this method can confirm only the existence of sturgeon species (in general) in fish ponds. In identifying residual DNA in the picogram level of the Persian sturgeon fish pond, SYBER Green real-time PCR method can be an acceptable method for barcoding with high efficiency compared to other methods such as conventional PCR method and non-invasive which is advantageous for the conservation of critically endangered sturgeons. | ||
| کلیدواژه‌ها | ||
| Aquatic ecosystem؛ Environmental DNA؛ Persian sturgeon؛ Real-time PCR | ||
| مراجع | ||
|
Abdolhay HA, Tahori HB. 2006. Fingerling production and release for stock enhancement of sturgeon in the Southern Caspian Sea: an overview. J Appl Ichthyol 22: 125-131. Barnes MA, Turner CR. 2016. The ecology of environmental DNA and implications for conservation genetics. Conserv Genet 17(1): 1-17. Beyraghdar Kashkooli O, Gröger J, Núñez-Riboni I. 2017. Qualitative assessment of climate-driven ecological shifts in the Caspian Sea. PloS One 12(5): e0176892. Birstein VJ, Bemis WE. 1997. How many species are there within the genus Acipenser? Environ Biol Fish 48: 157-163. Boscari E, Barmintseva A, Pujolar JM, Doukakis P, Mugue N, Congiu L. 2014. Species and hybrid identification of sturgeon caviar: a new molecular approach to detect illegal trade. Mol Ecol Resour 14(3): 489-498. Bronzi P, Rosenthal H. 2014. Present and future sturgeon and caviar production and marketing: A global market overview. J Appl Ichthyol 30(6): 1536-1546. Deiner K, Altermatt F. 2014. Transport distance of invertebrate environmental DNA in a natural river. PloS One 9(2): e88786. Deiner K, Bik HM, Mächler E, Seymour M, Lacoursière‐Roussel A, Altermatt F, ..., Bernatchez L. 2017. Environmental DNA metabarcoding: Transforming how we survey animal and plant communities. Mol Ecol 26(21): 5872-5895. Fazli H, Janbaz AA, Khedmati K. 2020. Risk of stock extinction in two species of kilkas (Clupeonella engrauliformis and C. grimmi) from the Caspian Sea. Iran J Ichthyol 7(1): 92-100. Goldberg CS, Sepulveda A, Ray A, Baumgardt J, Waits LP. 2013. Environmental DNA as a new method for early detection of New Zealand mudsnails (Potamopyrgus antipodarum). Freshw Sci 32(3): 792-800. Goldberg CS, Strickler KM, Fremier AK. 2018. Degradation and dispersion limit environmental DNA detection of rare amphibians in wetlands: Increasing efficacy of sampling designs. Sci Total Environ 633: 695-703. IUCN. 2021. The IUCN red list of threatened species. Available in: https: //www.iucnredlist.org/search (accessed 20 March 2021). Ivanov VP, Vlasenko AD, Khodorevskaya RP, Raspopov VM. 1999. Contemporary status of Caspiansturgeon (Acipenseridae) stock and its conservation. J Appl Ichthyol 15(4-5): 103-105. Jamshidi S, Monsef Shokri M, Hassanzadeh Saber M. 2021. Rapid detection of 4 species of Acipenser genus in the Caspian Sea Basin sturgeons and Siberian sturgeon using mitochondrial genome amplification method. J Fish 74(3): 351-362. Jerde CL, Olds BP, Shogren AJ, Andruszkiewicz EA, Mahon AR, Bolster D, …, Tank JL. 2016. Influence of stream bottom substrate on retention and transport of vertebrate environmental DNA. Environ Sci Technol 50(16): 8770-8779. Khodorevskaya RP, Ruban GJ, Pavlov DS. 2009. Behaviour, migrations, distribution, and stocks of sturgeons in the Volga-Caspian basin (No. 3). World Sturgeon Conservation Society: special publication No. 3. Books on Demand, Norderstedt, Germany. Kubista M, Andrade JM, Bengtsson M, Forootan A, Jonák J, Lind K, ..., Zoric N. 2006. The real-time polymerase chain reaction. Mol Aspects Med 27(2-3): 95-125. Ladell BA, Walleser LR, McCalla SG, Erickson RA, Amberg JJ. 2019. Ethanol and sodium acetate as a preservation method to delay degradation of environmental DNA. Conserv Genet Resour 11: 83-88. Lawson Handley L. 2015. How will the ‘molecular revolution’contribute to biological recording. Biol J Linn 115(3): 750-766. Li J, Lawson Handley LJ, Read DS, Hänfling B. 2018. The effect of filtration method on the efficiency of environmental DNA capture and quantification via metabarcoding. Mol Ecol Resour 18(5): 1102-1114. Lodge DM, Williams S, MacIsaac HJ, Hayes KR, Leung B, Reichard S, ..., McMichael A. 2006. Biological invasions: recommendations for US policy and management. Ecol Appl 16(6): 2035-2054. Ludwig A. 2008. Identification of Acipenseriformes species in trade. J Appl Ichthyol 24: 2-19. Moghim M, Kor D, Tavakolieshkalak M, Khoshghalb MB. 2006. Stock status of Persian Sturgeon (Acipenser persicus Borodin, 1897) along the Iranian coast of the Caspian Sea. J Appl Ichthyol 22: 99-107. Mugue NS, Barmintseva AE, Rastorguev SM, Mugue VN, Barmintsev VA. 2008. Polymorphism of the mitochondrial DNA control region in eight sturgeon species and development of a system for DNA-based species identification. Russ J Genet 44: 793-798. Pourang N, Eslami F, Nasrollahzadeh Saravi H, Fazli H. 2016. Strong biopollution Pourkazemi M. 2006. Caspian Sea sturgeon conservation and fisheries: past present and future. J Appl Ichthyol 22: 12-16. Rastorguev SM, Nedoluzhko AV, Mazur AM, Gruzdeva NM, Volkov AA, Barmintseva AE, ..., Prokhortchouk EB. 2013. High‐throughput SNP‐genotyping analysis of the relationships among P onto‐C aspian sturgeon species. Ecol Evol 3(8): 2612-2618. Sattari M. 2003. Parasites of Persian sturgeon (Acipenser persicus) from southwest of the Caspian Sea. Casp J Environ Sci 1(1): 61-66. Schenekar T, Schletterer M, Weiss SJ. 2020. Development of a TaqMan qPCR protocol for detecting Acipenser ruthenus in the Volga headwaters from eDNA samples. Conserv Genet Resour 12(3): 395-397. Taberlet P, Coissac E, Hajibabaei M, Rieseberg LH. 2012. Environmental DNA. Mol Ecol 21(8): 1789-1793. Tang Q, Liu H, Mayden R, Xiong B. 2006. Comparison of evolutionary rates in the mitochondrial DNA cytochrome b gene and control region and their implications for phylogeny of the Cobitoidea (Teleostei: Cypriniformes). Mol Phylogenet Evol 39(2): 347-357. Tavakoli M, Behrouz Khoshghalb MR, Kor D, Ghadirnejad H, Fadaee B, Joushideh H, ..., Taleshian H. 2010. Stock assessment of sturgeon fishes in the southern part of Caspian Sea (Iranian water). Iranian Fisheries Science Research Institute, Iran. Tavakoli M, Fazli H, Moghim M, Khoshghalb MR, Valinasab T, Abdolmaleki S. 2019. Population ecological parameters and stock assessment of Russian sturgeon Acipenser gueldenstaedti Brandt & Ratzeburg, 1833 in the Southern Caspian Sea. J Appl Ichthyol 35(1): 378-386. Thomsen PF, Willerslev E. 2015. Environmental DNA-an emerging tool in conservation for monitoring past and present biodiversity. Biol Conserv 183: 4-18. Vlasenko AD, Pavlov AV, Vasil’ev VP. 1989. Acipenser persicus Borodin, 1833. In: The Freshwater Fishes of Europe. Vol. 1. pt. II. General Introduction to Fishes. Acipenseriformes (ed. Holcik J), AULA-Verlag, Wiesbaden, Germany. Wang S, Yan Z, Hänfling B, Zheng X, Wang P, Fan J, Li J. 2021. Methodology of fish eDNA and its applications in ecology and environment. Sci Total Environ 755: 142622. doi: 10.1016/j.scitotenv.2020.142622. Waraniak JM, Blumstein DM, Scribner KT. 2017. Barcoding PCR primers detect larval lake sturgeon (Acipenser fulvescens) in diets of piscine predators. Conserv Genet Resour 10: 259-268. | ||
|
آمار تعداد مشاهده مقاله: 260 تعداد دریافت فایل اصل مقاله: 285 |
||