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تأثیر تمرین تناوبی با شدت بالا در شرایط نورموکسی و هایپوکسی بر بیان ژنهای دخیل در میتوفاژی کبد موشهای نر ویستار تحت رژیم غذایی پرچرب | ||
پژوهشنامه فیزیولوژی ورزشی کاربردی | ||
مقاله 5، دوره 18، شماره 36، اسفند 1401، صفحه 75-95 اصل مقاله (554.69 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22080/jaep.2024.26404.2169 | ||
نویسندگان | ||
فائقه قاسمی1؛ حمید محبی* 2 | ||
1دانشجوی دکتری فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران. | ||
2استاد گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران | ||
تاریخ دریافت: 13 آذر 1402، تاریخ بازنگری: 18 دی 1402، تاریخ پذیرش: 29 دی 1402 | ||
چکیده | ||
اهداف: مصرف رژیم غذایی پرچرب منجر به اختلال در عملکرد میتوکندری کبد و بروز اختلالات کبد چرب می شود و میتوفاژی که در حذف میتوکندریهای آسیب دیده نقش دارد احتمالاً بتواند در بهبود کبد چرب غیرالکلی مؤثر باشد. هدف از پژوهش حاضر، تعیین اثر تغذیه، تمرین و هایپوکسی بر میتوفاژی کبد در موش صحرایی نر ویستار بود. روش مطالعه: 32 سر موش صحرایی نر به طور تصادفی به چهار گروه 8تایی شامل گروههای رژیم غذایی نرمال (ND)، رژیم غذایی پرچرب (HFD)، رژیم غذایی پرچرب و تمرین در شرایط نورموکسی (HFD-HIIT)، رژیم غذایی پرچرب و تمرین در شرایط هایپوکسی (HFD-HHIIT) تقسیم شدند. پس از تعیین حداکثر سرعت هوازی (MAV) در شرایط نورموکسی (ارتفاع حدود 50 متر) و هایپوکسی-هیپوباریک (ارتفاع حدود 3000 متر)، پروتکلHIIT به مدت 12 هفته و 3 جلسه در هفته اجرا شد که شامل اجرای 3 تا 8 مرحله فعالیت 4 دقیقه ای با شدتی معادل 80 تا 93 درصد MAV و با دوره های استراحت فعال 2 دقیقه ای با شدت 50 درصد MAV بود. در پایان، سطوح بیان ژنهای HIF-1α، Parkin وFundc1 به روش RT-PCR اندازه گیری شد.. یافته ها: بیان ژنهای Parkin وFundc1 در دو گروه HFD+HIIT و HFD+HHIIT نسبت به HFD افزایش معنی داری داشت (0/05≥P). همچنین، بیان این دو ژن در گروه HFD-HHIIT افزایش معنی داری نسبت به گروه HFD-HIIT نشان داد (0/05≥P). محتوای چربی کبدی و بیان ژن HIF-1α در دو گروه HFD-HIIT و HFD-HHIIT نسبت به HFD کاهش معنی داری را نشان داد (0/05≥P). نتیجه گیری: به نظر می رسد، تمرین در شرایط هایپوکسی در مقایسه با نورموکسی تاثیر مازادی بر افزایش میتوفاژی کبد و به دنبال آن کنترل محتوای چربی کبدی دارد. | ||
کلیدواژهها | ||
تمرین تناوبی با شدت بالا؛ هایپوکسی؛ میتوفاژی؛ کبد چرب غیر الکلی؛ رژیم غذایی پرچرب | ||
عنوان مقاله [English] | ||
The effect of high intensity interval training in normoxia and hypobaric hypoxia situation on the expression of genes involved in liver mitophagy in male Wistar rats fed with high fat diet | ||
نویسندگان [English] | ||
Faeghe Ghasemi1؛ Hamid Mohebbi2 | ||
1Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran | ||
2Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Guilan, Rasht, Iran | ||
چکیده [English] | ||
Objectives: Consuming a high-fat diet leads to dysfunction of liver mitochondria and the occurrence of fatty liver disorders, and mitophagy, which plays a role in removing damaged mitochondria, can probably be effective in improving non-alcoholic fatty liver disease. The aim of this study was to determine the effect of nutrition, training and hypoxia on liver mitophagy in male Wistar rats Methods: Thirty-two male rats were randomly divided into four groups of eight including normal diet (ND), high-fat diet (HFD), high-fat diet and training in normoxia (HFD-HIIT) and high-fat diet and training in hypoxia (HFD-HHIIT). After determining the maximum aerobic velocity (MAV) in normoxia and hypoxia-hypobaric, the HIIT protocol was performed for 12 weeks and three sessions per week, which included 3 to 8 bouts 4-minute activity with an intensity of 80 to 93 percent of MAV and 2-minute active rest periods with an intensity of 50 percent of MAV. At the end, the levels of HIF-1α, Parkin and Fundc1 genes were measured through RT-PCR Results: The expression of Parkin and Fundc1 genes in HFD-HIIT and HFD-HHIIT groups increased significantly compared to HFD (P<0.05). Also, the HFD-HHIIT group showed a significant increase compared to the HFD-HIIT group (P<0.05). Also, liver fat content and HIF-1α gene expression showed a significant decrease in HFD-HIIT and HFD-HHIIT groups compared to HFD (P<0.05). Conclusion: It seems that training in hypoxic conditions compared to normoxia has an additional effect on increasing liver mitophagy and subsequently controlling liver fat content. | ||
کلیدواژهها [English] | ||
High Intensity Interval Training, Hypoxia, Mitophagy, NAFLD, High Fat Diet | ||
مراجع | ||
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